Sodium Selenite Improves In Vitro Maturation of Bos primigenius taurus Oocytes

被引：15

作者：

Martin Lizarraga, Raul ^{[1
]}

Mateo Anchordoquy, Juan ^{[1
,2
]}

Martin Galarza, Esteban ^{[1
,2
]}

Agustin Farnetano, Nicolas ^{[1
]}

Carranza-Martin, Ana ^{[1
]}

Cristina Furnus, Cecilia ^{[1
,3
]}

Alberto Mattioli, Guillermo ^{[1
,2
]}

Patricio Anchordoquy, Juan ^{[1
,2
]}

机构：

[1] UNLP, CONICET LA PLATA, IGEVET Inst Genet Vet Ingn Fernando N Dulout, Fac Ciencias Vet, Calle 60 & 118 S-N, RA-1900 La Plata, Buenos Aires, Argentina

[2] Univ Nacl La Plata, Fac Ciencias Vet, Fisiol, Calle 60 & 118 S-N, RA-1900 La Plata, Buenos Aires, Argentina

[3] Univ Nacl La Plata, Fac Ciencias Med Med, Citol Histol & Embriol, Calle 60 & 120 S-N, RA-1900 La Plata, Buenos Aires, Argentina

来源：

BIOLOGICAL TRACE ELEMENT RESEARCH | 2020年 / 197卷 / 01期

关键词：

Selenium; MDA; TAC; Apoptosis; GSH; Embryo development; GLUTATHIONE-PEROXIDASE ACTIVITY; INSULIN-TRANSFERRIN-SELENIUM; BOVINE OOCYTES; BLASTOCYST STAGE; DEVELOPMENTAL CAPACITY; SERUM-FREE; CELLS; SUPPLEMENTATION; ANTIOXIDANT; DEFICIENCY;

D O I：

10.1007/s12011-019-01966-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Selenium (Se) is an essential trace element with important functions in animals and whose deficiency is associated with reproductive failures. The aim of this study was to investigate the effect of Se concentrations during in vitro maturation (IVM) of Bos taurus oocyte within the reference ranges for Se status in cattle. For this purpose, Aberdeen Angus cumulus-oocyte complexes (COCs) were matured in IVM medium supplemented with 0, 10, 50, and 100 ng/mL Se (control, deficient, marginal, and adequate, respectively). The results demonstrated that marginal and adequate Se concentrations added during IVM increased viability and non-apoptotic cumulus cells (CC). Moreover, the addition of Se to culture media decreased malondialdehyde level in COC with all studied concentrations and increased total glutathione content in CC and oocytes with 10 ng/mL Se. On the other hand, total antioxidant capacity of COC, nuclear maturation, and the developmental capacity of oocytes were not modified by Se supplementation. However, 10 ng/mL Se increased hatching rate. In conclusion, supplementation with 10 ng/mL Se during in vitro maturation of Bos primigenius taurus oocytes should be considered to improve embryo quality.

引用

页码：149 / 158

页数：10

共 61 条

[1] Sodium selenite improves the in vitro follicular development by reducing the reactive oxygen species level and increasing the total antioxidant capacity and glutathione peroxide activity [J].