Rotation of microparticles with Bessel beams generated by diffractive elements

We show that imaging a non-diverging Bessel beam by a spherical lens leads to the generation of a diverging Bessel beam. Expressions for the projections of the Umov-Poynting vector for a two-dimensional TE-polarized Bessel beam and a three-dimensional paraxial linearly polarized Bessel beam are derived. A fifth-order Bessel beam is produced using a single optical element-a 16-level phase-only diffractive helical axicon fabricated using electron beam lithography. This beam was successfully used to trap and rotate 5-10 mum diameter yeast particles and polystyrene beads of diameter 5 mum.