A wideband picosecond pulsed electric fields (psPEF) exposure system for the nanoporation of biological cells

被引:6
|
作者
Gao, Mingxiang [1 ]
Xie, Yanzhao [1 ]
Wang, Siqi [1 ]
Shang, Sen [2 ]
Zhao, Jiping [2 ]
Lu, Xiaoyun [2 ]
机构
[1] Xi An Jiao Tong Univ, Sch Elect Engn, State Key Lab Elect Insulat & Power Equipment, Xian 710049, Peoples R China
[2] Xi An Jiao Tong Univ, Sch Life Sci & Technol, Dept Biol Sci & Bioengn, Key Lab Biomed Informat Engn,Minist Educ, Xian 710049, Peoples R China
关键词
Picosecond pulsed electric fields (psPEF); Grounded coplanar waveguide (GCPW); Biochip; Picosecond pulser; Nanoporation; Biological cells; MAMMALIAN-CELLS; ELECTROPORATION; PERTURBATION;
D O I
10.1016/j.bioelechem.2021.107790
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The effects and mechanisms of ultrashort and intense pulsed electric fields on biological cells remain some unknown. Especially for picosecond pulsed electric fields (psPEF) with a high pulse repetition rate, electroporation or nanoporation effects could be induced on cell membranes and intracellular organelle membranes. In this work, the design, implementation, and experimental validation of a wideband psPEF exposure system (WPES) is reported, comprising picosecond pulser and wideband biochip, for the in vitro exposure of suspended cells to high-intensity psPEF. Excited by repetitive picosecond pulses (the duration of 200 ps and the amplitude of a few kilovolts), the proposed biochip adopts grounded coplanar waveguide (GCPW) for a wide working bandwidth, which was fabricated with 160 mu m thick electrodes for uniform distribution of psPEF in the cross-section. To ensure that only psPEF is generated in the biological medium containing cells except for ionic current, this work proposes to install capillary tubes in the electrode gaps for electrical insulation and cells delivery. By electrical measurements in the time domain and frequency domain, the exposure system is adapted for local generation of extremely high intensity psPEF with the 3 dB bandwidth up to 4.2 GHz. Furthermore, biological experiments conducted on the developed exposure system verified its capability to permeabilize biological cells under the exposure of high-intensity psPEF. (C) 2021 Published by Elsevier B.V.
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页数:9
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