Alkaline phosphatase-responsive fluorescent polymer probe coated surface for colorimetric bacteria detection

被引：23

作者：

Kang, Eun Bi ^{[1
]}

Mazrad, Zihnil Adha Islamy ^{[2
]}

Robby, Akhmad Irhas ^{[2
]}

In, Insik ^{[2
,3
]}

Park, Sung Young ^{[1
,2
]}

机构：

[1] Korea Natl Univ Transportat, Dept Chem & Biol Engn, Chungju 380702, South Korea

[2] Korea Natl Univ Transportat, Dept IT Convergence, Chungju 380702, South Korea

[3] Korea Natl Univ Transportat, Dept Polymer Sci & Engn, Chungju 380702, South Korea

来源：

EUROPEAN POLYMER JOURNAL | 2018年 / 105卷

基金：

新加坡国家研究基金会;

关键词：

2-hydroxychalcone; Alkaline phosphatase; Enzymatic sensor; Bacteria detection; Antibacterial activity; AGGREGATION-INDUCED EMISSION; ASSAY; BIOSENSORS; PATHOGENS; ESIPT; STATE;

D O I：

10.1016/j.eurpolymj.2018.05.035

中图分类号：

O63 [高分子化学（高聚物）];

学科分类号：

070305 ; 080501 ; 081704 ;

摘要：

The present study aimed to develop an enzymatic colorimetric method using a surface-adsorbing biosensor to detect and kill bacteria in a single, simple, and rapid assay. The phosphorylated fluorescent probe 2-hydroxychalcone (HCAP) conjugated with an adhesive cationic polymer was designed (HCAP-PVP), which yielded greenish-yellow emission in aqueous buffer. Upon introduction of Escherichia coli and Staphylococcus aureus, the phosphate group inside the HCAP was cleaved by endogenous alkaline phosphatase (ALP) and the greenish yellow emission ratiometrically changed to deep-red emission. This biosensor system detected bacteria over a wide range of bacterial densities (10(1)-10(7) colony-forming units/mL) after 60 min, with similar bacterial detection abilities between aqueous- and solid-phase assays. Furthermore, the presence of a quaternary ammonium of dodecane in this system displayed efficient antibacterial activity because of the change in cellular hydrophobic interactions, which enabled this material to act as a dual sensor and killing material. Thus, this system is a novel, rapid, and simple enzymatic sensor with high sensitivity that can be used as a solid-based platform to detect and directly eliminate bacteria.

引用

页码：217 / 225

页数：9

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