Time-evolution of in vivo protein corona onto blood-circulating PEGylated liposomal doxorubicin (DOXIL) nanoparticles
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Hadjidemetriou, Marilena
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机构:Univ Manchester, Fac Med & Human Sci, Sch Med, Nanomed Lab, Manchester M13 9PT, Lancs, England
Hadjidemetriou, Marilena
Al-Ahmady, Zahraa
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机构:Univ Manchester, Fac Med & Human Sci, Sch Med, Nanomed Lab, Manchester M13 9PT, Lancs, England
Al-Ahmady, Zahraa
Kostarelos, Kostas
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Univ Manchester, Fac Med & Human Sci, Sch Med, Nanomed Lab, Manchester M13 9PT, Lancs, EnglandUniv Manchester, Fac Med & Human Sci, Sch Med, Nanomed Lab, Manchester M13 9PT, Lancs, England
Kostarelos, Kostas
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[1] Univ Manchester, Fac Med & Human Sci, Sch Med, Nanomed Lab, Manchester M13 9PT, Lancs, England
Nanoparticles (NPs) are instantly modified once injected in the bloodstream because of their interaction with the blood components. The spontaneous coating of NPs by proteins, once in contact with biological fluids, has been termed the 'protein corona' and it is considered to be a determinant factor for the pharmacological, toxicological and therapeutic profile of NPs. Protein exposure time is thought to greatly influence the composition of protein corona, however the dynamics of protein interactions under realistic, in vivo conditions remain unexplored. The aim of this study was to quantitatively and qualitatively investigate the time evolution of in vivo protein corona, formed onto blood circulating, clinically used, PEGylated liposomal doxorubicin. Protein adsorption profiles were determined 10 min, 1 h and 3 h post-injection of liposomes into CD-1 mice. The results demonstrated that a complex protein corona was formed as early as 10 min post-injection. Even though the total amount of protein adsorbed did not significantly change over time, the fluctuation of protein abundances observed indicated highly dynamic protein binding kinetics.
机构:
Univ Manchester, Fac Biol Med & Hlth, Nanomed Lab, Manchester, Lancs, EnglandUniv Manchester, Fac Biol Med & Hlth, Nanomed Lab, Manchester, Lancs, England
Hadjidemetriou, Marilena
Kostarelos, Kostas
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Univ Manchester, Fac Biol Med & Hlth, Nanomed Lab, Manchester, Lancs, EnglandUniv Manchester, Fac Biol Med & Hlth, Nanomed Lab, Manchester, Lancs, England
Kostarelos, Kostas
ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY,
2017,
253