Time-evolution of in vivo protein corona onto blood-circulating PEGylated liposomal doxorubicin (DOXIL) nanoparticles

被引:168
|
作者
Hadjidemetriou, Marilena
Al-Ahmady, Zahraa
Kostarelos, Kostas [1 ]
机构
[1] Univ Manchester, Fac Med & Human Sci, Sch Med, Nanomed Lab, Manchester M13 9PT, Lancs, England
基金
英国工程与自然科学研究理事会; 英国惠康基金;
关键词
TRIGGERED DRUG-RELEASE; TUMOR XENOGRAFT MODEL; PEG CHAIN-LENGTH; MILD HYPERTHERMIA; BIOMOLECULE CORONA; COMPLEMENT; SURFACE; ACTIVATION; DELIVERY; SERUM;
D O I
10.1039/c5nr09158f
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
Nanoparticles (NPs) are instantly modified once injected in the bloodstream because of their interaction with the blood components. The spontaneous coating of NPs by proteins, once in contact with biological fluids, has been termed the 'protein corona' and it is considered to be a determinant factor for the pharmacological, toxicological and therapeutic profile of NPs. Protein exposure time is thought to greatly influence the composition of protein corona, however the dynamics of protein interactions under realistic, in vivo conditions remain unexplored. The aim of this study was to quantitatively and qualitatively investigate the time evolution of in vivo protein corona, formed onto blood circulating, clinically used, PEGylated liposomal doxorubicin. Protein adsorption profiles were determined 10 min, 1 h and 3 h post-injection of liposomes into CD-1 mice. The results demonstrated that a complex protein corona was formed as early as 10 min post-injection. Even though the total amount of protein adsorbed did not significantly change over time, the fluctuation of protein abundances observed indicated highly dynamic protein binding kinetics.
引用
收藏
页码:6948 / 6957
页数:10
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  • [1] In vivo biomolecule corona onto clinically-used blood-circulating liposomes
    Hadjidemetriou, Marilena
    Kostarelos, Kostas
    ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 2017, 253