Quantitative structural organisation model for wheat endosperm cell walls: Cellulose as an important constituent

被引：69

作者：

Gartaula, Ghanendra ^{[1
]}

Dhital, Sushil ^{[1
]}

Netzel, Gabriele ^{[2
]}

Flanagan, Bernadine M. ^{[1
]}

Yakubov, Gleb E. ^{[3
]}

Beahan, Cherie T. ^{[4
]}

Collins, Helen M. ^{[5
]}

Burton, Rachel A. ^{[5
]}

Bacic, Antony ^{[4
,6
]}

Gidley, Michael J. ^{[1
]}

机构：

[1] Univ Queensland, ARC Ctr Excellence Plant Cell Walls, Ctr Nutr & Food Sci, Queensland Alliance Agr & Food Innovat, Hartley Teakle Bldg 83, St Lucia, Qld 4072, Australia

[2] Univ Queensland, Ctr Anim Sci, Queensland Alliance Agr & Food Innovat, Coopers Plains, Qld 4108, Australia

[3] Univ Queensland, Sch Chem Engn, ARC Ctr Excellence Plant Cell Walls, St Lucia, Qld 4072, Australia

[4] Univ Melbourne, Sch Biosci, ARC Ctr Excellence Plant Cell Walls, Parkville, Vic 3010, Australia

[5] Univ Adelaide, Sch Agr Food & Wine, ARC Ctr Excellence Plant Cell Walls, Waite Campus, Glen Osmond, SA 5064, Australia

[6] La Trobe Univ, La Trobe Inst Agr & Food LIAF, Sch Life Sci, Dept Anim Plant & Soil Sci,AgriBio, Bundoora, Vic 3086, Australia

来源：

CARBOHYDRATE POLYMERS | 2018年 / 196卷

基金：

澳大利亚研究理事会;

关键词：

Cell wall model; Wheat endosperm; Arabinoxylan; Mixed-linkage glucans; Cellulose; Diferulic acid; MECHANICAL-PROPERTIES; ACID COMPOSITION; FERULIC ACID; CROSS-LINKS; BARLEY; CARBOHYDRATE; ARABINOXYLAN; VARIABILITY; GRAIN; (1->3),(1->4)-BETA-D-GLUCAN;

D O I：

10.1016/j.carbpol.2018.05.041

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

The cell walls of cereal endosperms are a major source of fibre in many diets and of importance in seed structure and germination. Cell walls were isolated from both pure wheat endosperm and milled flour. C-13 CP/MAS NMR in conjunction with methylation analysis before and after acid hydrolysis showed that, in addition to arabinoxylan (AX) and (1, 3; 1, 4)-beta-D-glucan (MLG), wheat endosperm cell walls contain a significant proportion of cellulose (ca 20%) which is tightly bound to xylans and mannans. Light microscopy showed that the cellulose was relatively evenly distributed across the grain endosperm. The cell walls contain a fibrous acid-resistant core structure laminated by matrix polysaccharides as revealed by AFM imaging. A model for endosperm cell wall structural organisation is proposed, based on a core of cellulose and interacting non-cellulosic polysaccharides which anchors AX (with very occasional diferulic acid cross-linking) that in turn retains MLGs through physical entanglement.

引用

页码：199 / 208

页数：10

共 42 条

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