Physiological Versatility of the Extremely Thermoacidophilic Archaeon Metallosphaera sedula Supported by Transcriptomic Analysis of Heterotrophic, Autotrophic, and Mixotrophic Growth

被引：52
作者：
Auernik, Kathryne S. [1 ]
Kelly, Robert M. [1 ]
机构：
[1] N Carolina State Univ, Dept Chem & Biomol Engn, Raleigh, NC 27695 USA
来源：
APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2010年 / 76卷 / 03期
关键词：
3-HYDROXYPROPIONATE CYCLE; ENERGY-CONSERVATION; CARBON FIXATION; REDUCTASE; IDENTIFICATION; HYDROGENASES; COENZYME;
D O I：
10.1128/AEM.01336-09
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Comparative transcriptomic analysis of autotrophic, heterotrophic, and mixotrophic growth of the archaeon Metallosphaera sedula (70 degrees C, pH 2.0) revealed candidates for yet-to-be-confirmed components of the 3-hydroxypropionate/4-hydroxybutyrate pathway and implicated a membrane-bound hydrogenase (Msed_0944-Msed_0946) for growth on H-2. Routes for generation of ATP and reducing equivalents were also identified.
引用
收藏
页码：931 / 935
页数：5
相关论文
共 25 条
  • [1] Malonyl-coenzyme A reductase in the modified 3-hydroxypropionate cycle for autotrophic carbon fixation in archaeal Metallosphaera and Sulfolobus spp.
    Alber, Birgit
    Olinger, Marc
    Rieder, Annika
    Kockelkorn, Daniel
    Jobst, Bjoern
    Huegler, Michael
    Fuchs, Georg
    [J]. JOURNAL OF BACTERIOLOGY, 2006, 188 (24) : 8551 - 8559
  • [2] The genome sequence of the metal-mobilizing, extremely thermoacidophilic archaeon Metallosphaera sedula provides insights into bioleaching-associated metabolism
    Auernik, Kathryne S.
    Maezato, Yukari
    Blum, Paul H.
    Kelly, Robert M.
    [J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2008, 74 (03) : 682 - 692
  • [3] Identification of Components of Electron Transport Chains in the Extremely Thermoacidophilic Crenarchaeon Metallosphaera sedula through Iron and Sulfur Compound Oxidation Transcriptomes
    Auernik, Kathryne S.
    Kelly, Robert M.
    [J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2008, 74 (24) : 7723 - 7732
  • [4] A 3-hydroxypropionate/4-hydroxybutyrate autotrophic carbon dioxide assimilation pathway in archaea
    Berg, Ivan A.
    Kockelkorn, Daniel
    Buckel, Wolfgang
    Fuchs, Georg
    [J]. SCIENCE, 2007, 318 (5857) : 1782 - 1786
  • [5] They don't care
    Breithaupt, H
    [J]. EMBO REPORTS, 2001, 2 (01) : 1 - 1
  • [6] An archaeal b-type cytochrome containing a nonfunctional carbonic anhydrase-like domain
    Gomes, CG
    Kletzin, A
    Teixeira, M
    [J]. JOURNAL OF BIOLOGICAL INORGANIC CHEMISTRY, 2002, 7 (4-5): : 483 - 489
  • [7] A membrane-bound multienzyme, hydrogen-oxidizing, and sulfur-reducing complex from the hyperthermophilic bacterium Aquifex aeolicus
    Guiral, M
    Tron, P
    Aubert, C
    Gloter, A
    Iobbi-Nivol, C
    Giudici-Orticoni, MT
    [J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 2005, 280 (51) : 42004 - 42015
  • [8] Han CJ, 1998, BIOTECHNOL BIOENG, V58, P617, DOI 10.1002/(SICI)1097-0290(19980620)58:6<617::AID-BIT7>3.0.CO
  • [9] 2-L
  • [10] A dicarboxylate/4-hydroxybutyrate autotrophic carbon assimilation cycle in the hyperthermophilic Archaeum Ignicoccus hospitalis
    Huber, Harald
    Gallenberger, Martin
    Jahn, Ulrike
    Eylert, Eva
    Berg, Ivan A.
    Kockelkorn, Daniel
    Eisenreich, Wolfgang
    Fuchs, Georg
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2008, 105 (22) : 7851 - 7856
123