Bacterioplankton assemblages transforming dissolved organic compounds in coastal seawater

被引:51
|
作者
Mou, Xiaozhen [1 ]
Hodson, Robert E. [1 ]
Moran, Mary Ann [1 ]
机构
[1] Univ Georgia, Dept Marine Sci, Athens, GA 30602 USA
关键词
D O I
10.1111/j.1462-2920.2007.01318.x
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
To characterize bacterioplankton functional assemblages that transform specific components of the coastal seawater dissolved organic carbon (DOC) pool, bromodeoxyuridine (BrdU) was used to label the bacterioplankton cells that were active following addition of single-DOC model compounds: two organic osmolytes [dimethylsulfoniopropionate (DMSP) and glycine betaine (GIyB)] and two aromatic monomers [para-hydroxybenzoic acid (pHBA) and vanillic acid (VanA)]. Bacterial populations were analysed based on in situ fluorescent immunodetection of BrdU incorporation followed by fluorescence-activated cell sorting (FACS). Sorted cells were then characterized by 16S rDNA-based analysis. Populations with high BrdU incorporation level (HI) developed within 8 h of introduction of 100 nM model compound. Terminal restriction fragment length polymorphisms (T-RFLP) analysis indicated that the HI populations in all four amendments were composed of bacteria from the same major taxa (phylum and subphylum levels), but the relative abundance of each differed. High-resolution clone libraries (each containing similar to 200 clones) showed that the HI populations in the GIyB and VanA amendments consisted of both metabolic generalists and specialists within the alpha-Proteobacteria (mainly members of the Roseobacter clade), beta-Proteobacteria and gamma-Proteobacteria (mainly members of Altermonadaceae, Chromatiaceae, Oceanospirillaceae and Pseudomonadaceae). The presence of members of OM60/241, OM185, SAR11, SAR86 and SAR116 in the HI populations indicated that members of these groups can assimilate the model DOC compounds, providing some of the first glimpses into heterotrophy by members of these poorly understood environmental clusters.
引用
收藏
页码:2025 / 2037
页数:13
相关论文
共 50 条
  • [21] Temporal trends and identification of the sources of volatile organic compounds in coastal seawater
    Bravo-Linares, Claudio M.
    Mudge, Stephen M.
    JOURNAL OF ENVIRONMENTAL MONITORING, 2009, 11 (03): : 628 - 641
  • [22] Bacterioplankton assemblages in coastal ponds reflect the influence of hydrology and geomorphological setting
    Huggett, Megan J.
    Kavazos, Christopher R. J.
    Bernasconi, Rachele
    Czarnik, Robert
    Horwitz, Pierre
    FEMS MICROBIOLOGY ECOLOGY, 2017, 93 (06)
  • [23] Release of dissolved organic nitrogen by size-fractionated natural planktonic assemblages in coastal waters
    Hasegawa, T
    Koike, I
    Mukai, H
    MARINE ECOLOGY PROGRESS SERIES, 2000, 198 : 43 - 49
  • [24] Chemistry of dissolved organic matter in seawater
    Aluwihare, LI
    Repeta, DJ
    ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 1998, 216 : U351 - U351
  • [25] Dissolved organic matter in coastal rainwater: Concentration, bioavailability and depositional flux to seawater in southeastern China
    Bao, Hongyan
    Yi, Yueyuan
    Wang, Chao
    Spencer, Robert G. M.
    Deng, Xun
    Guo, Weidong
    MARINE CHEMISTRY, 2018, 205 : 48 - 55
  • [26] Community Composition and Diversity of Coastal Bacterioplankton Assemblages in Lakes Michigan, Erie, and Huron
    Ola A. Olapade
    Microbial Ecology, 2018, 75 : 598 - 608
  • [27] Growth of bacterioplankton and consumption of dissolved organic carbon in the Sargasso Sea
    Carlson, CA
    Ducklow, HW
    AQUATIC MICROBIAL ECOLOGY, 1996, 10 (01) : 69 - 85
  • [28] Community Composition and Diversity of Coastal Bacterioplankton Assemblages in Lakes Michigan, Erie, and Huron
    Olapade, Ola A.
    MICROBIAL ECOLOGY, 2018, 75 (03) : 598 - 608
  • [29] Production and loss of dissolved gaseous mercury in coastal seawater
    Amyot, M
    Gill, GA
    Morel, FMM
    ENVIRONMENTAL SCIENCE & TECHNOLOGY, 1997, 31 (12) : 3606 - 3611
  • [30] Removal rates of dissolved munitions compounds in seawater
    Smith, Richard W.
    Vlahos, Penny
    Tobias, Craig
    Ballentine, Mark
    Ariyarathna, Thivanka
    Cooper, Christopher
    CHEMOSPHERE, 2013, 92 (08) : 898 - 904