Enhanced secretion of substance P by cytokine-stimulated rat brain endothelium cultures

被引：29
作者：
Cioni, C
Renzi, D
Calabrò, A
Annunziata, P
机构：
[1] Univ Siena, Ist Sci Neurol, I-53100 Siena, Italy
[2] Univ Florence, Dept Gastroenterol, Florence, Italy
来源：
JOURNAL OF NEUROIMMUNOLOGY | 1998年 / 84卷 / 01期
关键词：
brain endothelium; neuropeptides; cytokines; blood-brain barrier; autoimmunity; inflammation;
D O I：
10.1016/S0165-5728(97)00235-X
中图分类号：
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
Substance P (SP) was analyzed in rat brain endothelium cultures after cytokine stimulation. SP secretion was found after stimulation with high doses of interleukin-1 beta (IL-1 beta) and tumor necrosis factor-alpha (TNF-alpha). High doses of interferon-gamma (INF-gamma) had no effect on this secretion. Elevated SP release was found to be associated with mRNA expression of beta-preprotachykinin (beta-PPT), precursor of SP, in the cells. Under cytokine stimulation, part of SP was bound to brain endothelial cell surface, suggesting the existence of an autocrine network for this neuropeptide. These findings suggest that SP map have an immunomodulatory action at the blood-brain barrier during inflammatory and autoimmune processes in the central nervous system. (C) 1998 Elsevier Science B.V.
引用
收藏
页码：76 / 85
页数：10
相关论文
共 26 条
[1]   SERUM ANTIBRAIN ENDOTHELIUM ANTIBODIES AND COGNITIVE ASSESSMENT IN PATIENTS WITH BINSWANGERS ENCEPHALOPATHY [J].
ANNUNZIATA, P ;
CIONI, C ;
MOSCHINI, F ;
RICCUCCI, A ;
GUAZZI, GC .
JOURNAL OF THE NEUROLOGICAL SCIENCES, 1995, 128 (01) :96-102
[2]   MODULATION OF HUMAN ENDOTHELIAL-CELL PERMEABILITY BY COMBINATIONS OF THE CYTOKINES INTERLEUKIN-1 ALPHA/BETA, TUMOR-NECROSIS-FACTOR-ALPHA AND INTERFERON-GAMMA [J].
BURKEGAFFNEY, A ;
KEENAN, AK .
IMMUNOPHARMACOLOGY, 1993, 25 (01) :1-9
[3]   The influence of cytokines on the integrity of the blood-brain barrier in vitro [J].
deVries, HE ;
BlomRoosemalen, MCM ;
vanOosten, M ;
deBoer, AG ;
vanBerkel, TJC ;
Breimer, DD ;
Kuiper, J .
JOURNAL OF NEUROIMMUNOLOGY, 1996, 64 (01) :37-43
[4]   PRODUCT ION OF THE CYTOKINES INTERLEUKIN-1 AND INTERLEUKIN-6 BY MURINE BRAIN MICROVESSEL ENDOTHELIUM AND SMOOTH-MUSCLE PERICYTES [J].
FABRY, Z ;
FITZSIMMONS, KM ;
HERLEIN, JA ;
MONINGER, TO ;
DOBBS, MB ;
HART, MN .
JOURNAL OF NEUROIMMUNOLOGY, 1993, 47 (01) :23-34
[5]   FUNCTIONAL NEUROKININ 1-RECEPTORS FOR SUBSTANCE-P ARE EXPRESSED BY HUMAN VASCULAR ENDOTHELIUM [J].
GREENO, EW ;
MANTYH, P ;
VERCELLOTTI, GM ;
MOLDOW, CF .
JOURNAL OF EXPERIMENTAL MEDICINE, 1993, 177 (05) :1269-1276
[6]   SUBSTANCE-P GENE-EXPRESSION IS REGULATED BY INTERLEUKIN-1 IN CULTURED SYMPATHETIC-GANGLIA [J].
HART, RP ;
SHADIACK, AM ;
JONAKAIT, GM .
JOURNAL OF NEUROSCIENCE RESEARCH, 1991, 29 (03) :282-291
[7]  
HELME RD, 1987, J IMMUNOL, V139, P3470
[8]   EFFECT OF NEUROPEPTIDES ON PRODUCTION OF INFLAMMATORY CYTOKINES BY HUMAN-MONOCYTES [J].
LOTZ, M ;
VAUGHAN, JH ;
CARSON, DA .
SCIENCE, 1988, 241 (4870) :1218-1221
[9]   Direct cell/cell contact with stimulated T lymphocytes induces the expression of cell adhesion molecules and cytokines by human brain microvascular endothelial cells [J].
Lou, J ;
Dayer, JM ;
Grau, GE ;
Burger, D .
EUROPEAN JOURNAL OF IMMUNOLOGY, 1996, 26 (12) :3107-3113
[10]  
LUBERNAROD J, 1994, J IMMUNOL, V152, P819
123