Calprotectin influences the aggregation of metal-free and metal-bound amyloid- β by direct interaction

被引：9

作者：

Lee, Hyuck Jin ^{[1
]}

Savelieff, Masha G. ^{[2
]}

Kang, Juhye ^{[1
,3
]}

Brophy, Megan Brunjes ^{[4
]}

Nakashige, Toshiki G. ^{[4
]}

Lee, Shin Jung C. ^{[3
]}

Nolan, Elizabeth M. ^{[4
]}

Lim, Mi Hee ^{[1
]}

机构：

[1] Korea Adv Inst Sci & Technol, Dept Chem, Daejeon 34141, South Korea

[2] SciGency Sci Commun, Ann Arbor, MI 48104 USA

[3] UNIST, Dept Chem, Ulsan 44919, South Korea

[4] MIT, Dept Chem, Cambridge, MA 02139 USA

来源：

METALLOMICS | 2018年 / 10卷 / 08期

基金：

新加坡国家研究基金会; 美国国家科学基金会;

关键词：

HUMAN SERUM-ALBUMIN; ALZHEIMERS-DISEASE; A-BETA; INFLAMMATORY S100A9; ZINC-BINDING; PEPTIDE; PROTEIN; AFFINITY; OLIGOMERS; CU(II);

D O I：

10.1039/c8mt00091c

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Proteins from the S100 family perform numerous functions and may contribute to Alzheimer's disease (AD). Herein, we report the effects of S100A8/S100A9 heterooligomer calprotectin (CP) and the S100B homodimer on metal-free and metal-bound amyloid- (A; A(40) and A(42)) aggregation in vitro. Studies performed with CP-Ser [S100A8(C42S)/S100A9(C3S) oligomer] indicate that the protein influences the aggregation profile for A(40) in both the absence and presence of metal ions [i.e., Zn(ii) and Cu(ii)]. Moreover, the detection of A(40)-CP-Ser complexes by mass spectrometry suggests a direct interaction as a possible mechanism for the involvement of CP in A(40) aggregation. Although the interaction of CP-Ser with A(40) impacts A(40) aggregation in vitro, the protein does not attenuate A-induced toxicity in SH-SY5Y cells. In contrast, S100B has a slight effect on the aggregation of A. Overall, this work supports a potential association of CP with A in the absence and presence of metal ions in AD.

引用

页码：1116 / 1127

页数：12

共 87 条

[11] Contributions of the S100A9 C-Terminal Tail to High-Affinity Mn(II) Chelation by the Host-Defense Protein Human Calprotectin [J].

Brophy, Megan Brunjes ;

Nakashige, Toshiki G. ;

Gaillard, Aleth ;

Nolan, Elizabeth M. .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2013, 135 (47) :17804-17817

[12] Calcium Ion Gradients Modulate the Zinc Affinity and Antibacterial Activity of Human Calprotectin [J].

Brophy, Megan Brunjes ;

Hayden, Joshua A. ;

Nolan, Elizabeth M. .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2012, 134 (43) :18089-18100

[13] The Multifactorial Nature of Alzheimer's Disease for Developing Potential Therapeutics [J].

Carmo Carreiras, M. ;

Mendes, Eduarda ;

Jesus Perry, M. ;

Francisco, Ana Paula ;

Marco-Contelles, J. .

CURRENT TOPICS IN MEDICINAL CHEMISTRY, 2013, 13 (15) :1745-1770

[14] Molecular Insights into Human Serum Albumin as a Receptor of Amyloid-β in the Extracellular Region [J].

Choi, Tae Su ;

Lee, Hyuck Jin ;

Han, Jong Yoon ;

Lim, Mi Hee ;

Kim, Hugh I. .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2017, 139 (43) :15437-15445

[15] Amyloid : one of three danger-associated molecules that are secondary inducers of the proinflammatory cytokines that mediate Alzheimer's disease [J].

Clark, I. A. ;

Vissel, B. .

BRITISH JOURNAL OF PHARMACOLOGY, 2015, 172 (15) :3714-3727

[16] High-resolution NMR studies of the zinc-binding site of the Alzheimer's amyloid β-peptide [J].

Danielsson, Jens ;

Pierattelli, Roberta ;

Banci, Lucia ;

Graslund, Astrid .

FEBS JOURNAL, 2007, 274 (01) :46-59

[17] Peripheral anti-Aβ antibody alters CNS and plasma Aβ clearance and decreases brain Aβ burden in a mouse model of Alzheimer's disease [J].

DeMattos, RB ;

Bales, KR ;

Cummins, DJ ;

Dodart, JC ;

Paul, SM ;

Holtzman, DM .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2001, 98 (15) :8850-8855

[18] A Redox-Active, Compact Molecule for Cross-Linking Amyloidogenic Peptides into Nontoxic, Off-Pathway Aggregates: In Vitro and In Vivo Efficacy and Molecular Mechanisms [J].

Derrick, Jeffrey S. ;

Kerr, Richard A. ;

Nam, Younwoo ;

Oh, Shin Bi ;

Lee, Hyuck Jin ;

Earnest, Kaylin G. ;

Suh, Nayoung ;

Peck, Kristy L. ;

Ozbil, Mehmet ;

Korshavn, Kyle J. ;

Ramamoorthy, Ayyalusamy ;

Prabhakar, Rajeev ;

Merino, Edward J. ;

Shearer, Jason ;

Lee, Joo-Yong ;

Ruotolo, Brandon T. ;

Lim, Mi Hee .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2015, 137 (46) :14785-14797

[19] Tools of the Trade: Investigations into Design Strategies of Small Molecules to Target Components in Alzheimer's Disease [J].

Derrick, Jeffrey S. ;

Lim, Mi Hee .

CHEMBIOCHEM, 2015, 16 (06) :887-898

[20] Misfolded proteins in Alzheimer's disease and type II diabetes [J].

DeToma, Alaina S. ;

Salamekh, Samer ;

Ramamoorthy, Ayyalusamy ;

Lim, Mi Hee .

CHEMICAL SOCIETY REVIEWS, 2012, 41 (02) :608-621

← 1 2 3 4 5 6 7 8 9 →