Medical-Grade Silicone Coated with Rhamnolipid R89 Is Effective against Staphylococcus spp. Biofilms

被引:36
作者
Ceresa, Chiara [1 ]
Tessarolo, Francesco [2 ,3 ]
Maniglio, Devid [2 ]
Tambone, Erica [1 ,2 ]
Carmagnola, Irene [4 ]
Fedeli, Emanuele [1 ]
Caola, Iole [5 ]
Nollo, Giandomenico [2 ,3 ]
Chiono, Valeria [4 ]
Allegrone, Gianna [1 ]
Rinaldi, Maurizio [1 ]
Fracchia, Letizia [1 ]
机构
[1] Univ Piemonte Orientale, Dept Pharmaceut Sci, I-28100 Novara, Italy
[2] Univ Trento, Dept Ind Engn, BIOtech Ctr Biomed Technol, I-38123 Trento, Italy
[3] Bruno Kessler Fdn, Healthcare Res & Innovat Program IRCS FBK PAT, I-38123 Trento, Italy
[4] Politecn Torino, Dept Mech & Aerosp Engn, I-10129 Turin, Italy
[5] Azienda Prov & Serv Sanitari Trento, Dept Med Lab, Sect Electron Microscopy, I-38123 Trento, Italy
来源
MOLECULES | 2019年 / 24卷 / 21期
关键词
biofilm; biosurfactants; Staphylococcus spp; antimicrobial; anti-adhesive; anti-biofilm; biomaterials; silicone; medical devices; CANDIDA-ALBICANS; PATHOGENIC BACTERIA; ADHESION; BIOSURFACTANTS; INFECTIONS; PREVENTION; SURFACES; DEVICES; MECHANISMS; STRATEGIES;
D O I
10.3390/molecules24213843
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Staphylococcus aureus and Staphylococcus epidermidis are considered two of the most important pathogens, and their biofilms frequently cause device-associated infections. Microbial biosurfactants recently emerged as a new generation of anti-adhesive and anti-biofilm agents for coating implantable devices to preserve biocompatibility. In this study, R89 biosurfactant (R89BS) was evaluated as an anti-biofilm coating on medical-grade silicone. R89BS is composed of homologues of the mono- (75%) and di-rhamnolipid (25%) families, as evidenced by mass spectrometry analysis. The antimicrobial activity against Staphylococcus spp. planktonic and sessile cells was evaluated by microdilution and metabolic activity assays. R89BS inhibited S. aureus and S. epidermidis growth with minimal inhibitory concentrations (MIC99) of 0.06 and 0.12 mg/mL, respectively and dispersed their pre-formed biofilms up to 93%. Silicone elastomeric discs (SEDs) coated by R89BS simple adsorption significantly counteracted Staphylococcus spp. biofilm formation, in terms of both built-up biomass (up to 60% inhibition at 72 h) and cell metabolic activity (up to 68% inhibition at 72 h). SEM analysis revealed significant inhibition of the amount of biofilm-covered surface. No cytotoxic effect on eukaryotic cells was detected at concentrations up to 0.2 mg/mL. R89BS-coated SEDs satisfy biocompatibility requirements for leaching products. Results indicate that rhamnolipid coatings are effective anti-biofilm treatments and represent a promising strategy for the prevention of infection associated with implantable devices.
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页数:17
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