HPLC analysis of metabolically produced formaldehyde

被引：11
作者：
Lengyel, J
Kaláisz, H
Szarvas, I
Peltz, C
Szarkáné-Bolehovszky, A
机构：
[1] Semmelweis Univ, H-1089 Budapest, Hungary
[2] Inst Isotopes Co Ltd, Budapest, Hungary
[3] Eotvos Lorand Univ, Dept Theoret Chem, Budapest, Hungary
来源：
JOURNAL OF CHROMATOGRAPHIC SCIENCE | 2003年 / 41卷 / 04期
基金：
匈牙利科学研究基金会;
关键词：
ALIPHATIC-ALDEHYDES; N-OXIDE; ACETALDEHYDE; URINE; EXCRETION; DEPRENYL; ETHANOL; ACETONE; BLOOD;
D O I：
10.1093/chromsci/41.4.177
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Radiolabelled (-)-deprenyl is orally administered to rats, and urinary elimination of radiolabelled formaldehyde is detected. The separation is performed using high-performance liquid chromatography on octadecyl-silica stationary phase. Both the radioactivity and the UV absorbance of the dinitrophenylhydrazine formaldehyde peak are determined. Formaldehyde generation takes place by N-demethylation. Low levels of formaldehyde may have a beneficial role in counterbalancing the oxidative stress of the everyday person's life.
引用
收藏
页码：177 / 181
页数：5
相关论文
共 19 条
  • [1] HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC METHOD FOR THE SIMULTANEOUS DETECTION OF MALONALDEHYDE, ACETALDEHYDE, FORMALDEHYDE, ACETONE AND PROPIONALDEHYDE TO MONITOR THE OXIDATIVE STRESS IN HEART
    CORDIS, GA
    BAGCHI, D
    MAULIK, N
    DAS, DK
    [J]. JOURNAL OF CHROMATOGRAPHY A, 1994, 661 (1-2) : 181 - 191
  • [2] Simultaneous determination of formaldehyde and methylglyoxal in urine: Involvement of semicarbazide-sensitive amine oxidase-mediated deamination in diabetic complications
    Deng, YL
    Yu, PH
    [J]. JOURNAL OF CHROMATOGRAPHIC SCIENCE, 1999, 37 (09) : 317 - 322
  • [3] MAPPING HUMAN-BRAIN MONOAMINE OXIDASE-A AND OXIDASE-B WITH C-11 LABELED SUICIDE INACTIVATORS AND PET
    FOWLER, JS
    MACGREGOR, RR
    WOLF, AP
    ARNETT, CD
    DEWEY, SL
    SCHLYER, D
    CHRISTMAN, D
    LOGAN, J
    SMITH, M
    SACHS, H
    AQUILONIUS, SM
    BJURLING, P
    HALLDIN, C
    HARTVIG, P
    LEENDERS, KL
    LUNDQVIST, H
    ORELAND, L
    STALNACKE, CG
    LANGSTROM, B
    [J]. SCIENCE, 1987, 235 (4787) : 481 - 485
  • [4] GAS-LIQUID-CHROMATOGRAPHIC ANALYSIS OF DIMEDONE DERIVATIVES OF FORMALDEHYDE AND OTHER ALIPHATIC-ALDEHYDES ON CAPILLARY COLUMNS
    JANOS, E
    BALLA, J
    TYIHAK, E
    GABORJANYI, R
    [J]. JOURNAL OF CHROMATOGRAPHY, 1980, 191 (APR): : 239 - 244
  • [5] Kalász H, 1998, ACTA BIOL HUNG, V49, P339
  • [6] TLC analysis of formaldehyde produced by metabolic N-demethylation
    Kalász, H
    Szarvas, T
    Szarkane-Bolehovszky, A
    Lengyel, J
    [J]. JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES, 2002, 25 (10-11) : 1589 - 1598
  • [7] Simultaneous determination of selegiline-N-oxide, a new indicator for selegiline administration, and other metabolites in urine by high-performance liquid chromatography-electrospray ionization mass spectrometry
    Katagi, M
    Tatsuno, M
    Miki, A
    Nishikawa, M
    Nakajima, K
    Tsuchihashi, H
    [J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 2001, 759 (01): : 125 - 133
  • [8] Simultaneous determination of dimethylamphetamine and its metabolites in rat hair by gas chromatography-mass spectrometry
    Kikura, R
    Nakahara, Y
    Kojima, S
    [J]. JOURNAL OF CHROMATOGRAPHY B, 2000, 741 (02): : 163 - 173
  • [9] Urinary excretion of deprenyl metabolites
    Lengyel, J
    Magyar, K
    Hollosi, I
    Bartok, T
    Bathori, M
    Kalasz, H
    Furst, S
    [J]. JOURNAL OF CHROMATOGRAPHY A, 1997, 762 (1-2) : 321 - 326
  • [10] DETERMINATION OF FREE ACETALDEHYDE IN BLOOD AS THE DINITROPHENYLHYDRAZONE DERIVATIVE BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY
    LUCAS, D
    MENEZ, JF
    BERTHOU, F
    PENNEC, Y
    FLOCH, HH
    [J]. JOURNAL OF CHROMATOGRAPHY, 1986, 382 : 57 - 66
12