Identification of the human homologue of mouse KIF4, a kinesin superfamily motor protein

被引：13
作者：
Oh, S
Hahn, H
Torrey, TA
Shin, H
Choi, W
Lee, YM
Morse, HC
Kim, W [1 ]
机构：
[1] Ajou Univ, Inst Med Sci, Suwon 442749, South Korea
[2] Ewha Womans Univ, Dept Biol Sci, Seoul 120750, South Korea
[3] NIAID, Immunopathol Lab, NIH, Bethesda, MD 20892 USA
来源：
BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION | 2000年 / 1493卷 / 1-2期
基金：
美国国家卫生研究院;
关键词：
kinesin; KIF4; cDNA library screening; reverse transcription polymerase chain reaction; genomic KIF4 gene; tissue-specific expression;
D O I：
10.1016/S0167-4781(00)00151-2
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A combination of RT-PCR with cDNA library screening led to the identification of the human homologue of murine KIF4 (mKIF4), a microtubule-based motor protein. The nucleotide sequence of human KIF4 (hKIF4) comprised part of the 5' untranslated region (UTR), a long open reading frame (ORF) encoding 1232 amino acids, and the entire 3' UTR. Homologies with mKIF4 were 82% and 85% for nucleotides and amino acids, respectively. A single transcript of 5.0 kb was highly expressed in hematopoietic tissues including fetal liver, spleen, and thymus, and adult thymus and bone marrow. Comparison of the ORF with that of mKIF4 revealed some dissimilarities in the putative cargo binding domain which might affect binding specificities. (C) 2000 Published by Elsevier Science B.V.
引用
收藏
页码：219 / 224
页数：6
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