Protective role of testis-specific peroxiredoxin 4 against cellular oxidative stress

被引：6

作者：

Tasaki, Eisuke ^{[1
]}

Matsumoto, Shotaro ^{[2
]}

Tada, Hisashi ^{[2
]}

Kurahashi, Toshihiro ^{[4
]}

Zhang, Xuhong ^{[5
]}

Fujii, Junichi ^{[5
]}

Utsumi, Toshihiko ^{[1
,2
,3
]}

Iuchi, Yoshihito ^{[1
,2
,3
]}

机构：

[1] Tottori Univ, United Grad Sch Agr, Dept Appl Bioresources Chem, 4-101 Koyamacho Minami, Tottori 6808553, Japan

[2] Yamaguchi Univ, Fac Agr, Dept Biol Chem, 1677-1 Yoshida, Yamaguchi 7538515, Japan

[3] Yamaguchi Univ, Grad Sch Sci & Technol Innovat, 1677-1 Yoshida, Yamaguchi 7538515, Japan

[4] Kyoto Prefectural Univ Med, Grad Sch Med Sci, Dept Pathol & Cell Regulat, Kamigyo Ku, 465 Kajii Cho, Kyoto 6028566, Japan

[5] Yamagata Univ, Grad Sch Med Sci, Dept Biochem & Mol Biol, 2-2-2 Iidanishi, Yamagata 9909585, Japan

来源：

JOURNAL OF CLINICAL BIOCHEMISTRY AND NUTRITION | 2017年 / 60卷 / 03期

基金：

日本学术振兴会;

关键词：

antioxidant activity; fluorescence image; oxidative stress; peroxiredoxin; ROS detection; ENDOPLASMIC-RETICULUM; CELLS; IV; OVEREXPRESSION; PROTEIN; ENZYME; H2O2;

D O I：

10.3164/jcbn.16-96

中图分类号：

R15 [营养卫生、食品卫生]; TS201 [基础科学];

学科分类号：

100403 ;

摘要：

Peroxiredoxin (PRDX), a newly discovered antioxidant enzyme, has an important role in hydrogen peroxide reduction. Among six PRDX genes (PRDX1-6) in mammals, PRDX4 gene is alternatively spliced to produce the somatic cell form (PRDX4) and the testis specific form (PRDX4t). In our previous study, PRDX4 knockout mice displayed testicular atrophy with an increase in cell death due to oxidative stress. However, the antioxidant function of PRDX4t is unknown. In this study, we demonstrate that PRDX4t plays a protective role against oxidative stress in the mammalian cell line HEK293T. The PRDX4t-EGFP plasmid was transferred into HEK293T cells; protein expression was confirmed in the cytoplasm. To determine the protective role of PRDX4t in cells, we performed image-based analysis of PRDX4t-EGFP expressed cells exposed to UV irradiation and hydrogen peroxide using fluorescent probe CelIROX. Our results suggested that PRDX4t-EGFP expressed cells had reduced levels of oxidative stress compared with cells that express only EGFP. This study highlights that PRDX4t plays an important role in cellular antioxidant defense.

引用

页码：156 / 161

页数：6

共 21 条

[1] Overexpression of catalase in cytosolic or mitochondrial compartment protects HepG2 cells against oxidative injury [J].