Free cyanide biodegradation by Fusarium solani, a fungi isolated from cyanide-contaminated soils, involves a cyanide hydrolyzing enzyme, cyanide hydratase (EC 4.2.1.66). This enzyme specifically seems to convert cyanhydric acid (HCN) to formamide but not the cyanide ion (CN-). Hence, the rate of free cyanide biodegradation is a function of the equilibrium HCN/CN- in solution. A better understanding of cyanide hydratase properties allows the definition of optimal conditions of F. solani biodegradation activity, and the proposition of a biological treatment of cyanide-contaminated alkaline soils and effluents.