Up-regulation of serine/threonine protein phosphatase type 2A regulatory subunits during methylprednisolone-induced differentiation of leukaemic HL-60 cells

被引：9

作者：

Aydin, HH

Selvi, N

Saydam, G

Tobu, M

Uzunoglu, S

Uslu, R

Buyukkececi, F

Omay, SB ^{[1
]}

机构：

[1] Ege Univ, Sch Med, Dept Hematol, TR-35100 Izmir, Turkey

[2] Ege Univ, Sch Med, Dept Biochem, TR-35100 Izmir, Turkey

[3] Ege Univ, Sch Med, Dept Med Oncol, TR-35100 Izmir, Turkey

[4] Celal Bayar Univ, Fac Sci & Art, Manisa, Turkey

来源：

CLINICAL AND LABORATORY HAEMATOLOGY | 2000年 / 22卷 / 05期

关键词：

PP2A; methylprednisolone; HL-60; cells; leukaemia; differentiation;

D O I：

10.1046/j.1365-2257.2000.00326.x

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Serine/threonine protein phosphatase 2A (PP2A) may play a role in leukaemic cell differentiation of the HL 60 myeloid leukaemic cell-line after methylprednisolone induction. We have investigated the specific enzyme activity and expression of catalytic and regulatory subunits of PP2A. The resulting specific enzyme activity and immunoblots showed an increase in enzyme activity and the expression of regulatory subunits after methylprednisolone treatment. There was no change in the expression of PP2A catalytic subunits. It is suggested that the effect of methylprednisolone on leukaemic differentiation may be the result of PP2A upregulation.

引用

收藏

页码：271 / 274

页数：4

相关论文

共 18 条

[1] SPECIFICITY OF THE POLYCATION-STIMULATED (TYPE-2A) AND ATP,MG-DEPENDENT (TYPE-1) PROTEIN PHOSPHATASES TOWARD SUBSTRATES PHOSPHORYLATED BY P34CDC2 KINASE [J].

AGOSTINIS, P ;

DERUA, R ;

SARNO, S ;

GORIS, J ;

MERLEVEDE, W .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1992, 205 (01) :241-248

[2] EXPRESSION OF LEUKOCYTE DIFFERENTIATION ANTIGENS DURING THE DIFFERENTIATION OF HL-60 CELLS INDUCED BY 1,25-DIHYDROXYVITAMIN D-3 - COMPARISON WITH THE MATURATION OF NORMAL MONOCYTIC AND GRANULOCYTIC BONE-MARROW CELLS [J].

BRACKMAN, D ;

LUNDJOHANSEN, F ;

AARSKOG, D .

JOURNAL OF LEUKOCYTE BIOLOGY, 1995, 58 (05) :547-555

[3] DIFFERENT OLIGOMERIC FORMS OF PROTEIN PHOSPHATASE-2A ACTIVATE AND INHIBIT SIMIAN-VIRUS 40 DNA-REPLICATION [J].

CEGIELSKA, A ;

SHAFFER, S ;

DERUA, R ;

GORIS, J ;

VIRSHUP, DM .

MOLECULAR AND CELLULAR BIOLOGY, 1994, 14 (07) :4616-4623

[4] THE STRUCTURE AND REGULATION OF PROTEIN PHOSPHATASES [J].

COHEN, P .

ANNUAL REVIEW OF BIOCHEMISTRY, 1989, 58 :453-508

[5]

ENCIO IJ, 1991, J BIOL CHEM, V266, P7182

[6] PROTEIN PHOSPHATASE 2A1 IS THE MAJOR ENZYME IN VERTEBRATE CELL-EXTRACTS THAT DEPHOSPHORYLATES SEVERAL PHYSIOLOGICAL SUBSTRATES FOR CYCLIN-DEPENDENT PROTEIN-KINASES [J].

FERRIGNO, P ;

LANGAN, TA ;

COHEN, P .

MOLECULAR BIOLOGY OF THE CELL, 1993, 4 (07) :669-677

[7] CDC55, A SACCHAROMYCES-CEREVISIAE GENE INVOLVED IN CELLULAR MORPHOGENESIS - IDENTIFICATION, CHARACTERIZATION, AND HOMOLOGY TO THE B-SUBUNIT OF MAMMALIAN TYPE-2A PROTEIN PHOSPHATASE [J].

HEALY, AM ;

ZOLNIEROWICZ, S ;

STAPLETON, AE ;

GOEBL, M ;

DEPAOLIROACH, AA ;

PRINGLE, JR .

MOLECULAR AND CELLULAR BIOLOGY, 1991, 11 (11) :5767-5780

[8] Morphologic evidence of apoptosis in childhood acute myeloblastic leukemia treated with high-dose methylprednisolone [J].

Hicsonmez, G ;

Erdemli, E ;

Tekelioglu, M ;

Tuncer, AM ;

Ozbek, N ;

Cetin, M ;

Cotter, TG .

LEUKEMIA & LYMPHOMA, 1996, 22 (1-2) :91-&

[9] CONTROL OF THE CDC2/CYCLIN-B COMPLEX IN XENOPUS EGG EXTRACTS ARRESTED AT A G2/M CHECKPOINT WITH DNA-SYNTHESIS INHIBITORS [J].

KUMAGAI, A ;

DUNPHY, WG .

MOLECULAR BIOLOGY OF THE CELL, 1995, 6 (02) :199-213

[10] THE ROLE OF PROTEIN PHOSPHATASE TYPE-2A IN THE XENOPUS CELL-CYCLE - INITIATION OF THE G2/M TRANSITION [J].

LEE, TH .

SEMINARS IN CANCER BIOLOGY, 1995, 6 (04) :203-209