Molecular cloning, genomic characterization and expression of novel human α1A-adrenoceptor isoforms

被引：68

作者：

Chang, DJ ^{[1
]}

Chang, TK ^{[1
]}

Yamanishi, SS ^{[1
]}

Salazar, FHR ^{[1
]}

Kosaka, AH ^{[1
]}

Khare, R ^{[1
]}

Bhakta, S ^{[1
]}

Jasper, JR ^{[1
]}

Shieh, IS ^{[1
]}

Lesnick, JD ^{[1
]}

Ford, APDW ^{[1
]}

Daniels, DV ^{[1
]}

Eglen, RM ^{[1
]}

Clarke, DE ^{[1
]}

Bach, C ^{[1
]}

Chan, HW ^{[1
]}

机构：

[1] Roche Biosci, Biol Res Ctr, Neurobiol Unit, Palo Alto, CA 94304 USA

来源：

FEBS LETTERS | 1998年 / 422卷 / 02期

关键词：

alpha(1)-adrenoceptor; splice variant; cDNA cloning; genome organization; expression;

D O I：

10.1016/S0014-5793(98)00024-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have isolated and characterized from human prostate novel splice variants of the human alpha(1A)-adrenoceptor, several of which generate truncated products and one isoform, alpha(1A-4), which has the identical splice site as the three previously described isoforms. Long-PCR on human genomic DNA showed that the alpha(1A-4) exon is located between those encoding the alpha(1A-1) and alpha(1A-3) variants. CHO-K1 cells stably expressing alpha(1A-4) showed ligand binding properties similar to those of the other functional isoforms as well as agonist-stimulated inositol phosphate accumulation. Quantitative PCR analyses revealed that alpha(1A-4) is the most abundant isoform expressed in the prostate with high levels also detected in liver and heart. (C) 1998 Federation of European Biochemical Societies.

引用

页码：279 / 283

页数：5

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