Separation of flavonol glycosides from Flaveria bidentis (L.) Kuntze by high-speed counter-current chromatography

被引：32
作者：
Xie, Qianqian [1 ]
Wei, Yun [1 ]
Zhang, Guoliang [2 ]
机构：
[1] Beijing Univ Chem Technol, State Key Lab Chem Resource Engn, Beijing 100029, Peoples R China
[2] Chinese Acad Agr Sci, Inst Environm & Sustainable Dev Agr, Beijing 100081, Peoples R China
来源：
SEPARATION AND PURIFICATION TECHNOLOGY | 2010年 / 72卷 / 02期
关键词：
High-speed counter-current chromatography; Flaveria bidentis (L.) Kuntze; Patuletin-3-O-glucoside; Hyperoside; Astragalin; LIQUID-CHROMATOGRAPHY; SULFATED FLAVONOIDS;
D O I：
10.1016/j.seppur.2010.02.012
中图分类号：
TQ [化学工业];
学科分类号：
0817 ;
摘要：
In order to utilize and control the invasive weed, Flaveria bidentis (L,) Kuntze, bioactive compounds mainly flavonoids from F. bidentis (L.) Kuntze were studied. High-speed counter-current chromatography (HSCCC) was successfully used for the separation of flavonol glycosides from F. bidentis (L.) Kuntze. The two-phase solvent system composed of ethyl acetate-methanol-water (10:0.4:10, v/v) was used for HSCCC. About 400 mg of the crude extract was separated by HSCCC, yielding 3.6 mg of patuletin-3-O-glucoside at a purity of over 97%; 4.4 mg of astragalin (kaempferol-3-O-glucoside) at a purity of over 98% and 4.5 mg of a mixture of hyperoside (quercetin-3-O-galactoside) and 6-methoxykaempferol-3-O-galactoside constituting over 97% of the fraction. The chemical structures were confirmed by MS and (1)H, (13)C, 1-D TOCSY NMR. (C) 2010 Elsevier B.V. All rights reserved.
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页码：229 / 233
页数：5
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