Assessment of cultured fish hepatocytes for studying cellular uptake and (eco)toxicity of nanoparticles

被引：18

作者：

Scown, Tessa M. ^{[1
]}

Goodhead, Rhys M. ^{[1
]}

Johnston, Blair D. ^{[1
]}

Moger, Julian ^{[2
]}

Baalousha, Mohammed ^{[3
]}

Lead, Jamie R. ^{[3
]}

van Aerle, Ronny ^{[1
]}

Iguchi, Taisen ^{[4
]}

Tyler, Charles R. ^{[1
]}

机构：

[1] Univ Exeter, Hatherly Labs, Ecotoxicol & Aquat Biol Res Grp, Exeter EX4 4PS, Devon, England

[2] Univ Exeter, Sch Phys, Biomed Phys Grp, Exeter EX4 4QL, Devon, England

[3] Univ Birmingham, Sch Geog Earth & Environm Sci, Birmingham B15 2TT, W Midlands, England

[4] Natl Inst Basic Biol, Okazaki Inst Integrat Biosci, Okazaki, Aichi 4448787, Japan

来源：

ENVIRONMENTAL CHEMISTRY | 2010年 / 7卷 / 01期

基金：

英国自然环境研究理事会;

关键词：

coherent anti-Stokes Raman scattering; in vitro; metal oxides; rainbow trout; silver; TITANIUM-DIOXIDE NANOPARTICLES; METAL-OXIDE NANOPARTICLES; TRANSMISSION ELECTRON-MICROSCOPY; TROUT ONCORHYNCHUS-MYKISS; HUMAN DERMAL FIBROBLASTS; WALLED CARBON NANOTUBES; FRESH-WATER FISH; RAINBOW-TROUT; IN-VITRO; OXIDATIVE STRESS;

D O I：

10.1071/EN09125

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Relatively little is known regarding the fate and possible toxic effects of engineered nanoparticles (ENPs) in the aquatic environment. We assessed the suitability of isolated trout hepatocytes for high throughput toxicity screening of ENPs, exposing them to a variety of metal and metal oxide nanoparticles and their bulk counterparts. We found no effects of the ENPs on cell viability, or on lipid peroxidation, with the exception of exposure to ZnO nanoparticles, or on glutathione-S-transferase (GST) levels, for exposure concentrations up to 500 mu g mL(-1). All ENPs, however, were internalised in the cultured hepatocytes, as shown by coherent anti-Stokes Raman scattering (CARS) as an imaging technique. Our findings suggest that fish hepatocyte cultures are suitable for studies investigating the cellular uptake of ENPs, but they do not appear to be sensitive to ENP exposure and thus not a good in vitro model for nanoparticle toxicity screening.

引用

页码：36 / 49

页数：14