Tumor suppressor microRNA-34a inhibits cell migration and invasion by targeting MMP-2/MMP-9/FNDC3B in esophageal squamous cell carcinoma

被引:59
作者
Yang, Lan [1 ,2 ]
Song, Xiaoyue [1 ,2 ]
Zhu, Jianbo [1 ,2 ,5 ]
Li, Mei [1 ,2 ]
Ji, Yu [1 ,2 ]
Wu, Fei [1 ,2 ]
Chen, Yunzhao [1 ,2 ]
Cui, Xiaobin [1 ,2 ]
Hu, Jianming [1 ,2 ]
Wang, Lianghai [1 ,2 ]
Cao, Yuwen [1 ,2 ]
Wei, Yutao [3 ]
Zhang, Wenjie [1 ,2 ]
Li, Feng [1 ,2 ,4 ]
机构
[1] Shihezi Univ, Sch Med, Dept Pathol, 59 North 2nd Rd, Shihezi 832002, Xinjiang, Peoples R China
[2] Shihezi Univ, Sch Med, Key Lab Xinjiang Endem & Ethn Dis, Shihezi, Xinjiang, Peoples R China
[3] Shihezi Univ, Sch Med, Affiliated Hosp 1, Shihezi, Xinjiang, Peoples R China
[4] Capital Med Univ, Beijing ChaoYang Hosp, Dept Pathol, Beijing, Peoples R China
[5] Third Mil Med Univ, Affiliated Hosp 2, Chongqing, Peoples R China
基金
中国国家自然科学基金;
关键词
esophageal squamous cell carcinoma; miR-34a; migration and invasion; matrix metalloproteinase 2/9; fibronectin type III domain containing 3B; CANCER STEM-CELLS; EPITHELIAL-MESENCHYMAL TRANSITION; ABERRANT CPG METHYLATION; BREAST-CANCER; GENE-EXPRESSION; DOWN-REGULATION; LUNG-CANCER; MIR-34A; PROLIFERATION; INACTIVATION;
D O I
10.3892/ijo.2017.4015
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
MicroRNAs (miRNAs) are a large family of small, non-coding RNAs that play a pivotal role in tumorigenesis. miR-34a, which is a member of the miR-34 family, is a downstream target of p53. Increasing evidence shows that miR-34a dysregulation may contribute to tumor development and progression in numerous cancers, including esophageal squamous cell carcinoma (ESCC). However, the mechanism of miR-34a in the regulation of ESCC cells need to be further elucidated because of the complex regulative network of miRNAs. The miR-34a expression in ESCC samples has been confirmed using quantitative reverse transcription polymerase chain reaction. The effects of miR-34a on cell migration and invasion were examined in ESCC cell lines using wound healing and Transwell assays, respectively. The effects of miR-34a on matrix metalloproteinase (MMP)-2 and -9 and fibronectin type III domain containing 3B (FNDC3B) expression levels were detected by luciferase reporter assays and western blot analysis. Quantitative polymerase chain reaction revealed that the miR-34a expression is significantly downregulated in the ESCC tissues compared to that in the adjacent normal tissues. miR-34a overexpression was significantly suppressed migration and invasion in the ESCC cells and simultaneously inhibited the MMP-2, MMP-9 and FNDC3B expression levels by targeting the coding and 3'-untranslated regions, respectively. The findings indicated that microRNA-34a suppresses cell migration and invasion by targeting MMP-2, MMP-9, and FNDC3B in ESCC.
引用
收藏
页码:378 / 388
页数:11
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