A longitudinal study of seroprevalence and seroconversion of Neospora caninum infection in dairy cattle in northeast Thailand

被引:14
作者
Chanlun, Aran
Emanuelson, Ulf
Frossling, Jenny
Aiumlamai, Suneerat
Bjorkman, Camilla
机构
[1] Swedish Univ Agr Sci, Dept Clin Sci, S-75007 Uppsala, Sweden
[2] Khon Kaen Univ, Fac Med Vet, Dept Med, Khon Kaen 40002, Thailand
[3] Natl Vet Inst, Dept Dis Control, S-75189 Uppsala, Sweden
[4] Khon Kaen Univ, Fac Med Vet, Dept Surg & Theriogenol, Khon Kaen 40002, Thailand
关键词
Neospora caninum; seroprevalence; seroconversion; Iscom ELISA; transmission; dairy cattle;
D O I
10.1016/j.vetpar.2007.02.008
中图分类号
R38 [医学寄生虫学]; Q [生物科学];
学科分类号
07 ; 0710 ; 09 ; 100103 ;
摘要
A long-term study was carried out in 11 dairy herds in the Khon Kaen province of northeast Thailand between August 2001 and November 2004. The objective was to investigate seroprevalence dynamics of Neospora caninum infection in the herds and to demonstrate patterns of seroconversion in individual cattle. Each herd was visited once a year, in total four times, and sera from cattle >3 months of age and farm dogs as well as a sample from the bulk milk were collected. All samples were analysed for presence of specific antibodies by an N. caninum iscom ELISA. The overall percentage of antibody-positive cattle was constant and varied only between 10 and 13% over the 4 years, but the variation in within-herd seroprevalence between herds was substantial. Two herds had >= 20% seropositive animals at all samplings and consistently high bulk milk OD, whereas two herds had no seropositive animal at the last two samplings and low bulk milk OD. Five herds had a decreasing trend of within-herd seroprevalence, whereas the remaining six herds had a higher portion of test-positive individuals at the end of the study. A total of 424 individuals were sampled more than once; 344 (81%) and 32 (8%) were consistently antibody-negative and antibody-positive, respectively. The proportions of animals that changed from being seronegative to seropositive and from being seropositive to seronegative between the years were 3.9-4.6% and 19-39%, respectively. Apparent vertical and horizontal transmission rates were 58% (95% CI; 44-71%) and 5% (95% CI; 3-7%), respectively. In conclusion, the overall percentage of N. caninum antibody-positive cattle was constant over the years, but the within-herd seroprevalence varied substantially between the herds. Seroconversions were likely to occur in individual cattle although most animals had consistent serological status throughout the study. (C) 2007 Elsevier B.V. All rights reserved.
引用
收藏
页码:242 / 248
页数:7
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