The C-terminal region of the Turnip mosaic virus P3 protein is essential for viral infection via targeting P3 to the viral replication complex

被引：48

作者：

Cui, Xiaoyan ^{[1
,2
,3
]}

Yaghmaiean, Hoda ^{[2
,3
,5
]}

Wu, Guanwei ^{[1
,2
,3
]}

Wu, Xiaoyan ^{[2
,4
]}

Chen, Xin ^{[1
]}

Thorn, Greg ^{[3
]}

Wang, Aiming ^{[2
,3
]}

机构：

[1] Jiangsu Acad Agr Sci, Inst Vegetable Crops, Nanjing 210014, Jiangsu, Peoples R China

[2] Agr & Agri Food Canada, London Res & Dev Ctr, London, ON N5V 4T3, Canada

[3] Western Univ, Dept Biol, London, ON N6A 5B7, Canada

[4] Zhejiang A&F Univ, Coll Agr & Food Sci, Linen 311300, Zhejiang, Peoples R China

[5] Univ British Columbia, Dept Bot, 3200-6270 Univ Blvd, Vancouver, BC V6T 1Z4, Canada

来源：

VIROLOGY | 2017年 / 510卷

基金：

中国国家自然科学基金; 加拿大自然科学与工程研究理事会;

关键词：

Plant RNA virus; Potyvirus; Turnip mosaic virus; P3; P3N-PIPO; Movement protein; Cell-to-cell movement; Punctate inclusion; Virus replication complex; PLUM-POX-VIRUS; ENDOPLASMIC-RETICULUM; RNA-POLYMERASE; FLUORESCENCE COMPLEMENTATION; MUTATIONAL ANALYSIS; PLANT-VIRUSES; POTYVIRUS; GENOME; GENE; RESISTANCE;

D O I：

10.1016/j.virol.2017.07.016

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Like other positive-strand RNA viruses, plant potyviruses assemble viral replication complexes (VRCs) on modified cellular membranes. Potyviruses encode two membrane proteins, 6K2 and P3. The former is known to play pivotal roles in the formation of membrane-associated VRCs. However, P3 remains to be one of the least characterized potyviral proteins. The P3 cistron codes for P3 as well as P3N-PIPO which results from RNA polymerase slippage. In this study, we show that the P3N-PIPO of Turnip mosaic virus (TuMV) is required for viral cell-to-cell movement but not for viral replication. We demonstrate that the C-terminal region of P3 (P3C) is indispensable for P3 to form cytoplasmic punctate inclusions and target VRCs. We reveal that TuMV mutants that lack P3C are replication-defective. Taken together, these data suggest that the P3 cistron has two distinct functions: P3N-PIPO as a dedicated movement protein and P3 as an essential component of the VRC.

引用

页码：147 / 155

页数：9

共 47 条

[1]

[Anonymous], 2016, SCI REP UK

[2]

[Anonymous], 2015, BIOMED RES INT, DOI [DOI 10.1371/J0URNAL.PGEN.1005164, DOI 10.1155/2015/238971]

[3] Visualization of the interaction between the precursors of VPg, the viral protein linked to the genome of Turnip mosaic virus, and the translation eukaryotic initiation factor iso 4E in planta [J].

Beauchemin, Chantal ;

Boutet, Nathalie ;

Laliberte, Jean-Francois .

JOURNAL OF VIROLOGY, 2007, 81 (02) :775-782

[4] Sumoylation of Turnip mosaic virus RNA Polymerase Promotes Viral Infection by Counteracting the Host NPR1-Mediated Immune Response [J].

Cheng, Xiaofei ;

Xiong, Ruyi ;

Li, Yinzi ;

Li, Fangfang ;

Zhou, Xueping ;

Wang, Aiming .

PLANT CELL, 2017, 29 (03) :508-525

[5] The Potyvirus Silencing Suppressor Protein VPg Mediates Degradation of SGS3 via Ubiquitination and Autophagy Pathways [J].

Cheng, Xiaofei ;

Wang, Aiming .

JOURNAL OF VIROLOGY, 2017, 91 (01)

[6] Visualizing double-stranded RNA distribution and dynamics in living cells by dsRNA binding-dependent fluorescence complementation [J].

Cheng, Xiaofei ;

Deng, Ping ;

Cui, Hongguang ;

Wang, Aiming .

VIROLOGY, 2015, 485 :439-451

[7] An internal RNA element in the P3 cistron of Wheat streak mosaic virus revealed by synonymous mutations that affect both movement and replication [J].

Choi, IR ;

Horken, KM ;

Stenger, DC ;

French, R .

JOURNAL OF GENERAL VIROLOGY, 2005, 86 :2605-2614

[8] Two separate regions in the genome of the Tobacco etch virus contain determinants of the wilting response of Tabasco pepper [J].

Chu, MH ;

LopezMoya, JJ ;

LlaveCorreas, C ;

Pirone, TP .

MOLECULAR PLANT-MICROBE INTERACTIONS, 1997, 10 (04) :472-480

[9] An overlapping essential gene in the Potyviridae [J].

Chung, Betty Y. -W. ;

Miller, W. Allen ;

Atkins, John F. ;

Firth, Andrew E. .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2008, 105 (15) :5897-5902

[10] Turnip Mosaic Virus RNA Replication Complex Vesicles Are Mobile, Align with Microfilaments, and Are Each Derived from a Single Viral Genome [J].

Cotton, Sophie ;

Grangeon, Romain ;

Thivierge, Karine ;

Mathieu, Isabelle ;

Ide, Christine ;

Wei, Taiyun ;

Wang, Aiming ;

Laliberte, Jean-Francois .

JOURNAL OF VIROLOGY, 2009, 83 (20) :10460-10471

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