Identification of Germline Stem Cells in the Ovary of the Teleost Medaka

被引：183
作者：
Nakamura, Shuhei [1 ]
Kobayashi, Kayo [1 ]
Nishimura, Toshiya [1 ,2 ]
Higashijima, Shin-ichi [3 ,4 ]
Tanaka, Minoru [1 ,2 ]
机构：
[1] Natl Inst Basic Biol, Lab Mol Genet Reprod, Okazaki, Aichi 4448787, Japan
[2] Grad Univ Adv Studies SOKENDAI, Dept Basic Biol, Okazaki, Aichi 4448585, Japan
[3] Natl Inst Nat Sci, Okazaki Inst Integrat Biosci, Natl Inst Physiol Sci, Okazaki, Aichi 4448787, Japan
[4] Grad Univ Adv Studies SOKENDAI, Dept Physiol Sci, Okazaki, Aichi 4448585, Japan
来源：
SCIENCE | 2010年 / 328卷 / 5985期
关键词：
ORYZIAS-LATIPES; HEMATOPOIETIC STEM; EXPRESSION; SOX9; OOGENESIS; REVEALS; FISH; MICE;
D O I：
10.1126/science.1185473
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Germline stem cells continually produce sperm in vertebrate testes, whereas there is no direct evidence showing that germline stem cells are present in adult vertebrate ovaries. By using transgenic methods and clonal analysis, we identified germline stem cells that supported oogenesis and the production of offspring in the ovaries of adult medaka fish. Early-stage germ cells were localized in clusters along interwoven threadlike cords of sox9b-expressing somatic cells (termed germinal cradles) where the germ cells developed. Germline stem cells gave rise to germ cells that divided to produce cysts, which then underwent cell death or separated to form follicles. Our results provide insight into the germline stem cell biology of medaka and provide a model system for studying vertebrate stem cell niches.
引用
收藏
页码：1561 / 1563
页数：3
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