Cationic lipids involved in gene transfer mobilize intracellular calcium

被引：10

作者：

Ouali, Mustapha ^{[1
]}

Ruysschaert, Jean-Marie ^{[1
]}

Lonez, Caroline ^{[1
]}

Vandenbranden, Michel ^{[1
]}

机构：

[1] Free Univ Brussels, Ctr Struct Biol & Bioinformat, Lab Struct & Funct Biol Membranes, B-1050 Brussels, Belgium

来源：

MOLECULAR MEMBRANE BIOLOGY | 2007年 / 24卷 / 03期

关键词：

cationic lipids; lipid/DNA complexes; intracellular Ca2+; phospholipase C; gene transfer;

D O I：

10.1080/09687860601108911

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Cationic lipids are efficient tools to introduce nucleic acids and proteins into cells. Elucidation of the mechanism and cellular pathways associated with such transport has been relatively tedious, even though significant progress has been made in the characterization of the intracellular trafficking of lipid/DNA complexes. Surprisingly little is known about the effects of these delivery vectors on cell functioning. In this report, we show that both cationic lipids and cationic lipid/DNA complexes mobilize the intracellular calcium. Removal of extracellular calcium did not significantly abolish this effect and preincubating cells with thapsigargin led to a decrease in [Ca2+](i), indicating that calcium was released mainly from internal calcium stores sensitive to thapsigargin. Pretreatment of the cells with the phospholipase C inhibitor U73122, blocked the [Ca2+](i) rise, suggesting an inositol dependent mechanism.

引用

页码：225 / 232

页数：8

共 24 条

[1]

BLEASDALE JE, 1989, ADV PROSTAG THROMB L, V19, P590

[2]

DEBS RJ, 1990, J BIOL CHEM, V265, P10189

[3] VESICLE FORMATION BY DOUBLE LONG-CHAIN AMIDINES [J].