Candida glabrata peroxiredoxins, Tsal and Tsa2, and sulfiredoxin, Srx1, protect against oxidative damage and are necessary for virulence

被引：22

作者：

Gutierrez-Escobedo, Guadalupe ^{[1
]}

Hernandez-Carreon, Oscar ^{[1
]}

Morales-Rojano, Brenda ^{[1
]}

Revuelta-Rodriguez, Brenda ^{[1
]}

Vazquez-Franco, Norma ^{[1
]}

Castano, Irene ^{[1
]}

De Las Penas, Alejandro ^{[1
]}

机构：

[1] Inst Potosino Invest Cient & Tecnol, IPICYT, Div Biol Mol, Camino Presa San Jose 2055,Col Lomas 4 Secc, San Luis Potosi 78216, San Luis Potosi, Mexico

来源：

FUNGAL GENETICS AND BIOLOGY | 2020年 / 135卷

关键词：

Candida glabrata; Tsa1; Tsa2; Srx1; Oxidative stress; Neutrophil; ALKYL HYDROPEROXIDE REDUCTASE; CYSTEINE-SULFINIC ACID; STRESS-RESPONSE; TRANSCRIPTION FACTORS; FUNGAL PATHOGEN; YEAST; RESISTANCE; EXPRESSION; GENE; PEROXIDASE;

D O I：

10.1016/j.fgb.2019.103287

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Candida glabrata is an opportunistic fungal pathogen that can cause life-threatening infections in immunocompromised patients. To ensure a successful infection, C. glabrata has evolved a variety of strategies to avoid killing within the host. One of these strategies is the resistance to oxidative stress. Here we show that the sulfiredoxin Srx1 and the peroxiredoxins, Tsa1 and Tsa2, are implicated in the oxidative stress response (OSR) and required for virulence. We analyzed null mutations in SRX1, TSA1 and TSA2 and showed that TSA2 and SRX1 are required to respond to oxidative stress. While TSA1 expression is constitutive, SRX1 and TSA2 are induced in the presence of H2O2 in a process dependent on H2O2 concentration and on both transcription factors Yap1 and Skn7. Msn2 and Msn4 are not necessary for the regulation of SRX1, TSA1 and TSA2. Interestingly, TSA1 and TSA2, which are localized in the cytoplasm, are induced in the presence of neutrophils and required for survival in these phagocytic cells.

引用

页数：15

共 67 条

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