Porous cellulose as promoter of oil production by the oleaginous yeast Lipomyces starkeyi using mixed agroindustrial wastes

被引:12
作者
Ganatsios, Vassilios [1 ,2 ]
Koutinas, Athanasios A. [1 ]
Bekatorou, Argyro [1 ]
Panagopoulos, Vassilios [1 ]
Banat, Ibrahim M. [3 ]
Terpou, Antonia [1 ]
Kopsahelis, Nikolaos [4 ]
机构
[1] Univ Patras, Dept Chem, Patras 26500, Greece
[2] Eastern Macedonia & Thrace Inst Technol, Dept Oenol & Beverage Technol, Kavala 66100, Greece
[3] Univ Ulster, Sch Biomed Sci, Coleraine BT52 1SA, Londonderry, North Ireland
[4] Agr Univ Athens, Dept Food Sci & Human Nutr, Iera Odos 75, GR-11855 Athens, Greece
关键词
Single cell oil; Lipomyces starkeyi; Cellulose; Immobilisation; Higher fatty acids; SOLID-STATE FERMENTATION; LIPID PRODUCTION; CO-FERMENTATION; BIODIESEL; MOLASSES; DESIGN; XYLOSE;
D O I
10.1016/j.biortech.2017.07.163
中图分类号
S2 [农业工程];
学科分类号
0828 ;
摘要
Enhanced single cell oil (SCO) production by the oleaginous yeast Lipomyces starkeyi DSM 70296, immobilised on delignified porous cellulose, is reported. Pure glucose media were initially used. The effects of substrate pH and treatment temperature were evaluated, showing that 30 degrees C and pH 5.0 were the optimum conditions for SCO production by the immobilised yeast. The immobilisation technique led to increased lipid accumulation and cell growth by 44% and 8%, respectively, in the glucose media, compared to free cells in suspension. This positive effect was also shown when low concentration mixed agro-industrial waste suspensions were used as substrates, leading to 85% enhanced SCO production in comparison with free cells. Higher fatty acid (HFA) analysis showed that yeast immobilisation led to increased formation of unsaturated HFAs (6%) and reduced saturated HFAs (5%) compared to free cells.
引用
收藏
页码:629 / 634
页数:6
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