Tandem conjugation of enzyme and antibody on silica nanoparticle for enzyme immunoassay

被引：25

作者：

Ke, Rongqin ^{[1
,2
]}

Yang, Wei ^{[1
,2
]}

Xia, Xiaohu ^{[1
,2
]}

Xu, Ye ^{[1
,2
]}

Li, Qingge ^{[1
,2
,3
]}

机构：

[1] Xiamen Univ, Mol Diagnost Lab, Dept Biomed Sci, Sch Life Sci, Xiamen 361005, Fujian, Peoples R China

[2] Xiamen Univ, Key Lab, Minist Educ Cell Biol & Tumor Cell Engn, Sch Life Sci, Xiamen 361005, Fujian, Peoples R China

[3] Key Lab Chem Biol Fujian, Xiamen 361005, Fujian, Peoples R China

来源：

ANALYTICAL BIOCHEMISTRY | 2010年 / 406卷 / 01期

基金：

中国国家自然科学基金;

关键词：

ELISA; Silica nanoparticles; Tandem conjugation; Hepatitis B surface antigen; LINKED-IMMUNOSORBENT-ASSAY; PROTEIN; CHEMILUMINESCENCE; CONSTRUCTION; LIPOSOMES; SYSTEMS; DESIGN;

D O I：

10.1016/j.ab.2010.06.039

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We present a new type of enzyme-antibody conjugate that simplifies the labeling procedure and increases the sensitivity of enzyme-linked immunosorbent assay (ELISA). The conjugates were prepared through layer-by-layer immobilization of enzyme and antibody on a silica nanoparticle scaffold. A maximal amount of enzyme was immobilized on the nanoparticle, followed by antibody linkage through Dextran 500. The conjugate could be easily purified from unreacted reagents by simple centrifugations. In comparison with the conventional antibody-enzyme conjugate used in ELISA, which often has one or two enzyme molecules per antibody, the new type of conjugate contained more enzyme molecules per antibody and provided a much higher signal and increased sensitivity. When used in an ELISA detection of the hepatitis B surface antigen (HBsAg), the detection limit was three times lower than that of the commercially available ELISA kit. (C) 2010 Elsevier Inc. All rights reserved.

引用

页码：8 / 13

页数：6

共 30 条

[1] Optimization of dye-doped silica nanoparticles prepared using a reverse microemulsion method [J].