Electrochemical Behavior of 1-Ferrocenyl-3-phenyl-2-propen-1-one on Glassy Carbon Electrode and Evaluation of its Interaction Parameters with DNA

The electrochemical behavior of 1-ferrocenyl-3-phenyl-2-propen-1-one (ferrocenylone) and its interaction with DNA was studied by a glassy carbon electrode using cyclic voltammetry (CV) technique. The results from CV were supported by UV-Visible spectroscopy performed under the similar conditions. The positive peak potential shift in CV and the bathochromic shift in the UV-Vis absorption spectra suggested an intercalative mode of binding. The binding constant (K = 1.39 +/- 0.02 x 10(4) mol(-1) L) and the binding site size (0.53 bp) were obtained from voltammetric data which leads to a standard Gibbs free energy change (Delta G(o) = -RT lnK) of -23.64 kJ mol(-1) and hence indicated the spontaneity of the binding interaction. The values of binding constants obtained from UV-Vis absorption and CV measurements, 1.26 +/- 0.01 x 10(4) and 1.39 +/- 0.02 x 10(4) mol(-1) L respectively, were in close agreement.