Investigations of retention rule in reversed-phase liquid chromatography - Effect of homologous series and column temperature on retention

A few linear retention equations including the various chromatographic parameters (for example, column temperature, composition of mobile phase, number of carbon atoms within the solute molecules, solvent molecules and alkyl ligand on bond phase surface) have been derived by using thermodynamic method based on both the displacement adsorption multi-interaction model in reversed-phase liquid, chromatography and the Martin equation. Both the linear retention rules and the experimental phenomena could be explained by these equations, and other linear retention rules which were not reported in the literature have also been predicted and verified experimentally.