Expression of the tobacco anionic peroxidase gene is tissue-specific and developmentally regulated

被引:40
|
作者
Klotz, KL [1 ]
Liu, TTY [1 ]
Liu, L [1 ]
Lagrimini, LM [1 ]
机构
[1] Ohio State Univ, Dept Hort & Crop Sci, Columbus, OH 43210 USA
关键词
developmental expression; lignin; Nicotiana sylvestris; Nicotiana tabacum; peroxidase; tissue-specific expression;
D O I
10.1023/A:1005939600344
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Transcriptionally regulated expression of tobacco anionic peroxidase was investigated with regard to tissue specificity and developmental regulation. Two tobacco species, Nicotiana sylvestris and Nicotiana tabacum cv. Xanthi, were stably transformed with a gene chimera composed of 3 kb of the tobacco anionic peroxidase promoter, the Escherichia coli beta-glucuronidase (GUS) coding region and the nopaline synthase terminator. Gene expression was regulated spatially and developmentally in all organs, and generally increased with age and maturity of the plant, tissue or organ. In the aerial portions of the plant, GUS activity was strongly expressed in trichomes and epidermis at nearly all developmental stages. In later stages of development, activity was also detected in ground tissue and parenchyma cells associated with vascular tissues. Activity in roots was limited to cortical cells and vascular-associated parenchyma cells. In reproductive tissue, expression was observed in sepals and petals before anthesis, and in all floral organs after anthesis. Expression was never detected in vascular tissue and was poorly correlated with lignification except in the cells surrounding primary xylem and pericyclic fibers in N. sylvestris. These studies suggest that this peroxidase isoenzyme is only limitedly involved in lignification but may be important in plant defense, growth and development.
引用
收藏
页码:509 / 520
页数:12
相关论文
共 50 条
  • [21] Characterization of a tissue-specific and developmentally regulated β-1,3-glucanase gene family in Prunus persica
    Ko, TS
    Lee, SM
    Schaefer, SC
    Korban, SS
    PLANT PHYSIOLOGY AND BIOCHEMISTRY, 2003, 41 (11-12) : 955 - 963
  • [22] TISSUE-SPECIFIC AND DEVELOPMENTALLY SPECIFIC CONTROLS INVOLVED IN RAT ALDOLASE-B GENE-EXPRESSION
    TSUTSUMI, K
    TSUTSUMI, R
    DAIMON, M
    NUMAZAKI, M
    ISHIKAWA, K
    ISOZYMES-CURRENT TOPICS IN BIOLOGICAL AND MEDICAL RESEARCH, 1987, 14 : 177 - 193
  • [23] TISSUE-SPECIFIC AND DEVELOPMENTALLY REGULATED TRANSCRIPTION OF THE INSULIN-LIKE GROWTH FACTOR-II GENE
    GRAY, A
    TAM, AW
    DULL, TJ
    HAYFLICK, J
    PINTAR, J
    CAVENEE, WK
    KOUFOS, A
    ULLRICH, A
    DNA-A JOURNAL OF MOLECULAR & CELLULAR BIOLOGY, 1987, 6 (04): : 283 - 295
  • [24] Characterization of a tissue-specific and developmentally regulated β-1,3-glucanase gene in pea (Pisum sativum)
    Peter Buchner
    Christine Rochat
    Sylvie Wuillème
    Jean-Pierre Boutin
    Plant Molecular Biology, 2002, 49 : 171 - 186
  • [25] IDENTIFICATION OF A TISSUE-SPECIFIC, DEVELOPMENTALLY REGULATED ENHANCER ELEMENT IN THE MURINE CARDIAC TROPONIN-C GENE
    PARMACEK, MS
    VORA, AJ
    LEIDEN, JM
    CLINICAL RESEARCH, 1990, 38 (02): : A471 - A471
  • [26] Characterization of a tissue-specific and developmentally regulated β-1,3-glucanase gene in pea (Pisum sativum)
    Buchner, P
    Rochat, C
    Wuillème, S
    Boutin, JP
    PLANT MOLECULAR BIOLOGY, 2002, 49 (02) : 171 - 186
  • [27] Tissue-specific expression of the rolA gene mediates morphological changes in transgenic tobacco
    Guivarch, A
    Carneiro, M
    Vilaine, F
    Pautot, V
    Chriqui, D
    PLANT MOLECULAR BIOLOGY, 1996, 30 (01) : 125 - 134
  • [28] Tissue-specific expression and promoter analysis of the tobacco Itp1 gene
    Canevascini, S
    Caderas, D
    Mandel, T
    Fleming, AJ
    Dupuis, I
    Kuhlemeier, C
    PLANT PHYSIOLOGY, 1996, 112 (02) : 513 - 524
  • [29] Tissue-specific gene expression and ecdysone-regulated genomic networks in Drosophila
    Li, TR
    White, KP
    DEVELOPMENTAL CELL, 2003, 5 (01) : 59 - 72
  • [30] FOLATE RECEPTOR GENE-EXPRESSION IS TISSUE-SPECIFIC AND TEMPORALLY-REGULATED
    WILLIS, SA
    LACEY, SW
    WEITMAN, SD
    KAMEN, BA
    NISEN, PD
    PEDIATRIC RESEARCH, 1991, 29 (04) : A56 - A56