Development of a test to discriminate between Foot- and Mouth Disease Virus (FMDV)-infected and vaccinated cattle

被引：0

作者：

Höhlich, BJ

Gerner, W

Wiesmüller, KH

Schlapp, T

Haas, B

Glatthaar-Saalmüller, B

Straub, OC

Pfaff, E

Saalmüller, A

机构：

[1] Bundesforschung Anstalt Viruskrankheiten Tiere, Inst Immunol, D-72076 Tubingen, Germany

[2] EMC Microcollect, D-72070 Tubingen, Germany

[3] Bayer Anim Hlth, D-51368 Leverkusen, Germany

[4] Labor Dr Glatthaar, D-72770 Reutlingen, Germany

来源：

TIERARZTLICHE UMSCHAU | 2003年 / 58卷 / 04期

关键词：

FMDV; diagnosis; peptide-ELISA; B-cell epitopes;

D O I：

暂无

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

The use of overlapping 15mer synthetic peptides, covering the whole open reading frame of FMDV strain OX in a peptide ELISA, allowed the identification of 12 FMDV strain OX-specific linear B-cell epitopes. Six of these linear B-cell epitopes, located in the nonstructural proteins were used in further assays to compare the reactivity of sera from vaccinated and infected cattle. Antibodies recognizing these peptides could be detected only in sera derived from infected cattle. In further experiments the reactivity of the six peptides with sera from animals infected with different strains of FMDV was tested and strain independent infection-specific epitopes were identified. These results clearly demonstrate a simple peptide-based assay to discriminate between infected and conventionally FMD-vaccinated animals.

引用

页码：171 / 178

页数：8

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