Influence of free energy on the attachment of Salmonella Enteritidis and Listeria monocytogenes on stainless steels AISI 304 and AISI 316

被引：21

作者：

Casarin, Leticia Sopena ^{[1
,5
]}

Casarin, Fabricio de Oliveira ^{[2
]}

Brandelli, Adriano ^{[1
]}

Novello, Junia ^{[1
,3
]}

Ferreira, Sukarno Olavo ^{[4
]}

Tondo, Eduardo Cesar ^{[1
]}

机构：

[1] Univ Fed Rio Grande do Sul, ICTA, Lab Microbiol & Controle Alimentos, Av Bento Goncalves 9500-43212, BR-91501970 Porto Alegre, RS, Brazil

[2] Univ Fed Rio Grande do Sul, Programa Posgrad Ciencia Mat, Av Bento Goncalves 9500, BR-91501970 Porto Alegre, RS, Brazil

[3] UCS, Ctr Ciencias Exatas & Tecnol, Rua Francisco Getulio Vargas 1130, BR-95070560 Caxias Do Sul, RS, Brazil

[4] Univ Fed Vicosa, Dept Fis, Ave Peter Henry Rolfs,S-N Campus Univ, BR-36570900 Vicosa, MG, Brazil

[5] UFCSPA, Dept Nutr, Av Sarmento Leite 245, BR-90050170 Porto Alegre, RS, Brazil

来源：

LWT-FOOD SCIENCE AND TECHNOLOGY | 2016年 / 69卷

关键词：

Stainless steel; Bacterial adhesion; Listeria monocytogenes; Salmonella Enteritidis; Free energy; BIOFILM FORMATION; STAPHYLOCOCCUS-AUREUS; ADHESION; SURFACES; RESISTANCE; TEMPERATURE; POPULATION; SURVIVAL; GROWTH; RUBBER;

D O I：

10.1016/j.lwt.2016.01.035

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

Bacterial attachment to stainless steels AISI (American Iron and Steel Institute) 304 and 316, the preferred material choices for food equipment, is a very important food safety consideration. The purpose of the present study was to investigate the attachment of two important food pathogens on commercial samples of these surfaces focusing on the influence of material topography characterized by Atomic Force Microscopy and considering the hydrophobicity and free energy of interaction, determined by measuring contact angle and application of thermodynamic theory principles. Results showed that S. Enteritidis and Listeria monocytogenes were able to attach to samples of stainless steels, however, initially (t = 0), the number of adhered S. Enteritidis (2.55 log CFU/cm(2)) was higher than L. monocytogenes (1.68 logCFU/cm(2)). Corroborating these results, the adhesion was thermodynamically more favorable for S. Enteritidis. Even though, the number of adhered cells were similar on both stainless steels samples, negative total energy was higher on AISI 316, for both bacteria. Moreover, the surface's roughness (0.032 pm and 0.021 gm) and hydrophobicity of cells and materials did not show a positive correlation with bacterial adherence. (c) 2016 Published by Elsevier Ltd.

引用

页码：131 / 138

页数：8

共 45 条

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