An improved indirect ELISA for specific detection of antibodies against classical swine fever virus based on structurally designed E2 protein expressed in suspension mammalian cells

被引:18
|
作者
Ji, Shengwei [1 ,2 ]
Luo, Yuzi [1 ]
Zhang, Tengteng [1 ,2 ]
Shao, Lina [1 ]
Meng, Xing-Yu [1 ]
Wang, Yimin [1 ]
Gao, Yao [1 ]
Li, Yongfeng [1 ]
Li, Su [1 ]
Sun, Yuan [1 ]
Jin, Xin [2 ]
Qiu, Hua-Ji [1 ]
机构
[1] Chinese Acad Agr Sci, HVRI, State Key Lab Vet Biotechnol, 678 Haping Rd, Harbin 150069, Heilongjiang, Peoples R China
[2] Yanbian Univ, Coll Agr, Dept Anim Med, Gongyuan St, Yanji 133002, Peoples R China
基金
中国国家自然科学基金;
关键词
VIRAL DIARRHEA VIRUS; HOG-CHOLERA VIRUS; ENVELOPE GLYCOPROTEIN E2; RANDOM PEPTIDE LIBRARY; MONOCLONAL-ANTIBODIES; DIFFERENT PESTIVIRUSES; LINEAR EPITOPE; STRAIN BRESCIA; PIGS; INFECTIONS;
D O I
10.1007/s00705-018-3809-7
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Classical swine fever (CSF), which is caused by classical swine fever virus (CSFV), is a highly contagious disease of pigs. CSFV is genetically and serologically related to bovine viral diarrhea virus (BVDV), a ruminant pestivirus. However, currently available ELISAs based on the full-length E2 protein of CSFV cannot discriminate anti-CSFV from anti-BVDV antibodies. In this study, a truncated CSFV E2 protein (amino acids 690 to 879) covering antigenic domains B/C/D/A (E2(B/C/D/A)) was designed based on homologous modeling according to the crystal structure of the BVDV E2 protein. The E2(B/C/D/A) protein was expressed in CHO cells adapted to serum-free suspension culture, and an indirect ELISA (iELISA) was established based on the recombinant protein. No serological cross-reaction was observed for anti-BVDV sera in the iELISA. When testing 282 swine serum samples, the iELISA displayed a high sensitivity (119/127, 93.7%) and specificity (143/155, 92.3%), with an agreement of 92.9% (262/282) and 92.2% (260/282) with virus neutralization test and the IDEXX CSFV blocking ELISA, respectively. Taken together, the newly developed iELISA is highly specific and sensitive and able to differentiate anti-CSFV from anti-BVDV antibodies.
引用
收藏
页码:1831 / 1839
页数:9
相关论文
共 50 条
  • [31] Immunogenicity of a lettuce-derived vaccine candidate expressing the E2 protein against classical swine fever virus
    Yiu, Jinn-Chin
    Liu, Cheng-Wei
    Su, Ruei-Yuan
    Lai, Wan-Jun
    Tseng, Menq-Jiau
    Ou, Sheng-Jung
    PLANT CELL TISSUE AND ORGAN CULTURE, 2013, 113 (03) : 483 - 490
  • [32] Immunogenicity of a lettuce-derived vaccine candidate expressing the E2 protein against classical swine fever virus
    Jinn-Chin Yiu
    Cheng-Wei Liu
    Ruei-Yuan Su
    Wan-Jun Lai
    Menq-Jiau Tseng
    Sheng-Jung Ou
    Plant Cell, Tissue and Organ Culture (PCTOC), 2013, 113 : 483 - 490
  • [33] Development of Plant-produced E2 Protein for Use as a Green Vaccine Against Classical Swine Fever Virus
    Sohn, Eun-Ju
    Lee, Yongjik
    Park, Namjo
    Park, Minhee
    Kim, Nam Hyung
    Park, Soohong
    Min, Kyungmin
    Gu, Sungmin
    Park, Youngmin
    Song, Jaeyoung
    An, Dong-jun
    Hwang, Inhwan
    JOURNAL OF PLANT BIOLOGY, 2018, 61 (04) : 241 - 252
  • [34] Development of Plant-produced E2 Protein for Use as a Green Vaccine Against Classical Swine Fever Virus
    Eun-Ju Sohn
    Yongjik Lee
    Namjo Park
    Minhee Park
    Nam Hyung Kim
    Soohong Park
    Kyungmin Min
    Sungmin Gu
    Youngmin Park
    Jaeyoung Song
    Dong-jun An
    Inhwan Hwang
    Journal of Plant Biology, 2018, 61 : 241 - 252
  • [35] Gold nanoparticles enhance immune responses in mice against recombinant classical swine fever virus E2 protein
    Li, Yafei
    Jin, Qianyue
    Ding, Peiyang
    Zhou, Wen
    Chai, Yongxiao
    Li, Xufeng
    Wang, Yao
    Zhang, Gaiping
    BIOTECHNOLOGY LETTERS, 2020, 42 (07) : 1169 - 1180
  • [36] Construction of recombinant swinepox viruses and expression of the classical swine fever virus E2 protein
    Hahn, J
    Park, SH
    Song, JY
    An, SH
    Ahn, BY
    JOURNAL OF VIROLOGICAL METHODS, 2001, 93 (1-2) : 49 - 56
  • [37] Enhancing expression of the classical swine fever virus glycoprotein E2 in yeast and its application to a blocking ELISA
    Cheng, Chih-Yuan
    Wu, Ching-Wei
    Lin, Guang-Jan
    Lee, Wei-Cheng
    Chien, Maw-Sheng
    Huang, Chienjin
    JOURNAL OF BIOTECHNOLOGY, 2014, 174 : 1 - 6
  • [38] Development of an Indirect ELISA to Detect African Swine Fever Virus pp62 Protein-Specific Antibodies
    Zhong, Kexin
    Zhu, Mengmeng
    Yuan, Qichao
    Deng, Zhibang
    Feng, Simeng
    Liu, Daoxin
    Yuan, Xiaomin
    FRONTIERS IN VETERINARY SCIENCE, 2022, 8
  • [39] Establishment and application of a solid-phase blocking ELISA method for detection of antibodies against classical swine fever virus
    Cao, Yuying
    Yuan, Li
    Yang, Shunli
    Shang, Youjun
    Yang, Bin
    Jing, Zhizhong
    Guo, Huichen
    Yin, Shuanghui
    JOURNAL OF VETERINARY SCIENCE, 2022, 23 (05)
  • [40] GLYCOPROTEIN E2 OF CLASSICAL SWINE FEVER VIRUS - EXPRESSION IN INSECT CELLS AND IDENTIFICATION AS A RIBONUCLEASE
    HULST, MM
    HIMES, G
    NEWBIGIN, ED
    MOORMANN, RJM
    VIROLOGY, 1994, 200 (02) : 558 - 565