Analysis of multispectral imaging with the AstroPath platform informs efficacy of PD-1 blockade

被引：136

作者：

Berry, Sneha ^{[1
,2
,3
,4
,14
]}

Giraldo, Nicolas A. ^{[5
]}

Green, Benjamin F. ^{[1
,2
,3
,6
,14
]}

Cottrell, Tricia R. ^{[5
,15
]}

Stein, Julie E. ^{[5
]}

Engle, Elizabeth L. ^{[1
,2
,3
,6
]}

Xu, Haiying ^{[1
,2
,3
,6
]}

Ogurtsova, Aleksandra ^{[1
,2
,3
,6
]}

Roberts, Charles ^{[2
,3
,6
]}

Wang, Daphne ^{[6
]}

Nguyen, Peter ^{[6
]}

Zhu, Qingfeng ^{[5
]}

Soto-Diaz, Sigfredo ^{[2
,3
,6
]}

Loyola, Jose ^{[2
,3
,6
]}

Sander, Inbal B. ^{[6
]}

Wong, Pok Fai ^{[7
]}

Jessel, Shlomit ^{[7
]}

Doyle, Joshua ^{[8
,9
]}

Signer, Danielle ^{[6
]}

Wilton, Richard ^{[8
,9
]}

Roskes, Jeffrey S. ^{[8
,9
]}

Eminizer, Margaret ^{[8
,9
]}

Park, Seyoun ^{[1
,10
]}

Sunshine, Joel C. ^{[6
]}

Jaffee, Elizabeth M. ^{[1
,2
,3
,4
]}

Baras, Alexander ^{[5
]}

De Marzo, Angelo M. ^{[4
,5
]}

Topalian, Suzanne L. ^{[2
,3
,11
]}

Kluger, Harriet ^{[12
]}

Cope, Leslie ^{[1
,2
,3
,13
]}

Lipson, Evan J. ^{[1
,2
,3
,4
]}

Danilova, Ludmila ^{[1
,2
,3
,13
]}

Anders, Robert A. ^{[1
,2
,3
,5
]}

Rimm, David L. ^{[7
]}

Pardoll, Drew M. ^{[1
,2
,3
,4
]}

Szalay, Alexander S. ^{[1
,8
,9
]}

Taube, Janis M. ^{[1
,2
,3
,5
,6
]}

机构：

[1] Johns Hopkins Univ, Mark Fdn Ctr Adv Genom & Imaging, Baltimore, MD 21287 USA

[2] Johns Hopkins Univ, Bloomberg Kimmel Inst Canc Immunotherapy, Baltimore, MD 21287 USA

[3] Johns Hopkins Univ, Sidney Kimmel Comprehens Canc Ctr, Baltimore, MD 21287 USA

[4] Johns Hopkins Univ, Dept Oncol, Sch Med, Baltimore, MD 21287 USA

[5] Johns Hopkins Univ, Dept Pathol, Sch Med, Baltimore, MD 21287 USA

[6] Johns Hopkins Univ, Dept Dermatol, Sch Med, Baltimore, MD 21287 USA

[7] Yale Univ, Dept Pathol, Sch Med, New Haven, CT 06510 USA

[8] Johns Hopkins Univ, Dept Astron & Phys, Baltimore, MD 21218 USA

[9] Johns Hopkins Univ, Inst Data Intens Engn & Sci, Baltimore, MD 21218 USA

[10] Johns Hopkins Univ, Dept Radiol, Sch Med, Baltimore, MD 21287 USA

[11] Johns Hopkins Univ, Dept Surg, Sch Med, Baltimore, MD 21287 USA

[12] Yale Univ, Dept Med, Div Med Oncol, Sch Med, New Haven, CT 06510 USA

[13] Johns Hopkins Univ, Sidney Kimmel Comprehens Canc Ctr, Div Biostat & Bioinformat, Sch Med, Baltimore, MD 21287 USA

[14] Akoya Biosci, Marlborough, MA 01752 USA

[15] Queens Univ, Dept Pathol & Mol Med, Kingston, ON K7L 3N6, Canada

来源：

SCIENCE | 2021年 / 372卷 / 6547期

关键词：

T-CELLS; MULTIPLEX IMMUNOHISTOCHEMISTRY; IMMUNE MICROENVIRONMENT; CANCER; IMMUNOFLUORESCENCE; CHECKPOINTS; EXPRESSION; SUBSET; TUMORS;

D O I：

10.1126/science.aba2609

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Next-generation tissue-based biomarkers for immunotherapy will likely include the simultaneous analysis of multiple cell types and their spatial interactions, as well as distinct expression patterns of immunoregulatory molecules. Here, we introduce a comprehensive platform for multispectral imaging and mapping of multiple parameters in tumor tissue sections with high-fidelity single-cell resolution. Image analysis and data handling components were drawn from the field of astronomy. Using this "AstroPath" whole-slide platform and only six markers, we identified key features in pretreatment melanoma specimens that predicted response to anti-programmed cell death-1 (PD-1)-based therapy, including CD163(+)PD-L1(-) myeloid cells and CD8(+)FoxP3(+)PD-1(low/mid) T cells. These features were combined to stratify long-term survival after anti-PD-1 blockade. This signature was validated in an independent cohort of patients with melanoma from a different institution.

引用

页码：1166 / +

页数：54

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