Analysis of multispectral imaging with the AstroPath platform informs efficacy of PD-1 blockade

被引：127

作者：

Berry, Sneha ^{[1
,2
,3
,4
,14
]}

Giraldo, Nicolas A. ^{[5
]}

Green, Benjamin F. ^{[1
,2
,3
,6
,14
]}

Cottrell, Tricia R. ^{[5
,15
]}

Stein, Julie E. ^{[5
]}

Engle, Elizabeth L. ^{[1
,2
,3
,6
]}

Xu, Haiying ^{[1
,2
,3
,6
]}

Ogurtsova, Aleksandra ^{[1
,2
,3
,6
]}

Roberts, Charles ^{[2
,3
,6
]}

Wang, Daphne ^{[6
]}

Nguyen, Peter ^{[6
]}

Zhu, Qingfeng ^{[5
]}

Soto-Diaz, Sigfredo ^{[2
,3
,6
]}

Loyola, Jose ^{[2
,3
,6
]}

Sander, Inbal B. ^{[6
]}

Wong, Pok Fai ^{[7
]}

Jessel, Shlomit ^{[7
]}

Doyle, Joshua ^{[8
,9
]}

Signer, Danielle ^{[6
]}

Wilton, Richard ^{[8
,9
]}

Roskes, Jeffrey S. ^{[8
,9
]}

Eminizer, Margaret ^{[8
,9
]}

Park, Seyoun ^{[1
,10
]}

Sunshine, Joel C. ^{[6
]}

Jaffee, Elizabeth M. ^{[1
,2
,3
,4
]}

Baras, Alexander ^{[5
]}

De Marzo, Angelo M. ^{[4
,5
]}

Topalian, Suzanne L. ^{[2
,3
,11
]}

Kluger, Harriet ^{[12
]}

Cope, Leslie ^{[1
,2
,3
,13
]}

Lipson, Evan J. ^{[1
,2
,3
,4
]}

Danilova, Ludmila ^{[1
,2
,3
,13
]}

Anders, Robert A. ^{[1
,2
,3
,5
]}

Rimm, David L. ^{[7
]}

Pardoll, Drew M. ^{[1
,2
,3
,4
]}

Szalay, Alexander S. ^{[1
,8
,9
]}

Taube, Janis M. ^{[1
,2
,3
,5
,6
]}

机构：

[1] Johns Hopkins Univ, Mark Fdn Ctr Adv Genom & Imaging, Baltimore, MD 21287 USA

[2] Johns Hopkins Univ, Bloomberg Kimmel Inst Canc Immunotherapy, Baltimore, MD 21287 USA

[3] Johns Hopkins Univ, Sidney Kimmel Comprehens Canc Ctr, Baltimore, MD 21287 USA

[4] Johns Hopkins Univ, Dept Oncol, Sch Med, Baltimore, MD 21287 USA

[5] Johns Hopkins Univ, Dept Pathol, Sch Med, Baltimore, MD 21287 USA

[6] Johns Hopkins Univ, Dept Dermatol, Sch Med, Baltimore, MD 21287 USA

[7] Yale Univ, Dept Pathol, Sch Med, New Haven, CT 06510 USA

[8] Johns Hopkins Univ, Dept Astron & Phys, Baltimore, MD 21218 USA

[9] Johns Hopkins Univ, Inst Data Intens Engn & Sci, Baltimore, MD 21218 USA

[10] Johns Hopkins Univ, Dept Radiol, Sch Med, Baltimore, MD 21287 USA

[11] Johns Hopkins Univ, Dept Surg, Sch Med, Baltimore, MD 21287 USA

[12] Yale Univ, Dept Med, Div Med Oncol, Sch Med, New Haven, CT 06510 USA

[13] Johns Hopkins Univ, Sidney Kimmel Comprehens Canc Ctr, Div Biostat & Bioinformat, Sch Med, Baltimore, MD 21287 USA

[14] Akoya Biosci, Marlborough, MA 01752 USA

[15] Queens Univ, Dept Pathol & Mol Med, Kingston, ON K7L 3N6, Canada

来源：

SCIENCE | 2021年 / 372卷 / 6547期

关键词：

T-CELLS; MULTIPLEX IMMUNOHISTOCHEMISTRY; IMMUNE MICROENVIRONMENT; CANCER; IMMUNOFLUORESCENCE; CHECKPOINTS; EXPRESSION; SUBSET; TUMORS;

D O I：

10.1126/science.aba2609

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Next-generation tissue-based biomarkers for immunotherapy will likely include the simultaneous analysis of multiple cell types and their spatial interactions, as well as distinct expression patterns of immunoregulatory molecules. Here, we introduce a comprehensive platform for multispectral imaging and mapping of multiple parameters in tumor tissue sections with high-fidelity single-cell resolution. Image analysis and data handling components were drawn from the field of astronomy. Using this "AstroPath" whole-slide platform and only six markers, we identified key features in pretreatment melanoma specimens that predicted response to anti-programmed cell death-1 (PD-1)-based therapy, including CD163(+)PD-L1(-) myeloid cells and CD8(+)FoxP3(+)PD-1(low/mid) T cells. These features were combined to stratify long-term survival after anti-PD-1 blockade. This signature was validated in an independent cohort of patients with melanoma from a different institution.

引用

页码：1166 / +

页数：54

共 47 条

[1] Inhibitory receptors and ligands beyond PD-1, PD-L1 and CTLA-4: breakthroughs or backups
Andrews, Lawrence P.
Yano, Hiroshi
Vignali, Dario A. A.
[J]. NATURE IMMUNOLOGY, 2019, 20 (11) : 1425 - 1434
[2] Tumor-Reactive CD8+ Early Effector T Cells Identified at Tumor Site in Primary and Metastatic Melanoma
Anichini, Andrea
Molla, Alessandra
Vegetti, Claudia
Bersani, Ilaria
Zappasodi, Roberta
Arienti, Flavio
Ravagnani, Fernando
Maurichi, Andrea
Patuzzo, Roberto
Santinami, Mario
Pircher, Hanspeter
Di Nicola, Massimo
Mortarini, Roberta
[J]. CANCER RESEARCH, 2010, 70 (21) : 8378 - 8387
[3] B7-H3 Expression in Merkel Cell Carcinoma-Associated Endothelial Cells Correlates with Locally Aggressive Primary Tumor Features and Increased Vascular Density
Aung, Phyu P.
Parra, Edwin Roger
Barua, Souptik
Sui, Dawen
Ning, Jing
Mino, Barbara
Ledesma, Debora Alejandra
Curry, Jonathan L.
Nagarajan, Priyadharsini
Torres-Cabala, Carlos A.
Efstathiou, Eleni
Hoang, Anh G.
Wong, Michael K.
Wargo, Jennifer A.
Lazar, Alexander J.
Rao, Arvind
Prieto, Victor G.
Wistuba, Ignacio
Tetzlaff, Michael T.
[J]. CLINICAL CANCER RESEARCH, 2019, 25 (11) : 3455 - 3467
[4] Selective expansion of a subset of exhausted CD8 T cells by αPD-L1 blockade
Blackburn, Shawn D.
Shin, Haina
Freeman, Gordon J.
Wherry, E. John
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2008, 105 (39) : 15016 - 15021
[5] Transcriptional Basis of Mouse and Human Dendritic Cell Heterogeneity
Brown, Chrysothemis C.
Gudjonson, Herman
Pritykin, Yuri
Deep, Deeksha
Lavallee, Vincent-Philippe
Mendoza, Alejandra
Fromme, Rachel
Mazutis, Linas
Ariyan, Charlotte
Leslie, Christina
Pe'er, Dana
Rudensky, Alexander Y.
[J]. CELL, 2019, 179 (04) : 846 - +
[6] Elements of cancer immunity and the cancer-immune set point
Chen, Daniel S.
Mellman, Ira
[J]. NATURE, 2017, 541 (7637) : 321 - 330
[7] A dynamic CD2-rich compartment at the outer edge of the immunological synapse boosts and integrates signals
Demetriou, Philippos
Abu-Shah, Enas
Valvo, Salvatore
McCuaig, Sarah
Mayya, Viveka
Kvalvaag, Audun
Starkey, Thomas
Korobchevskaya, Kseniya
Lee, Lennard Y. W.
Friedrich, Matthias
Mann, Elizabeth
Kutuzov, Mikhail A.
Morotti, Matteo
Wietek, Nina
Rada, Heather
Yusuf, Shamsideen
Afrose, Jehan
Siokis, Anastasios
Meyer-Hermann, Michael
Ahmed, Ahmed Ashour
Depoil, David
Dustin, Michael L.
[J]. NATURE IMMUNOLOGY, 2020, 21 (10) : 1232 - +
[8] Principles of Analytic Validation of Immunohistochemical Assays Guideline From the College of American Pathologists Pathology and Laboratory Quality Center
Fitzgibbons, Patrick L.
Bradley, Linda A.
Fatheree, Lisa A.
Alsabeh, Randa
Fulton, Regan S.
Goldsmith, Jeffrey D.
Haas, Thomas S.
Karabakhtsian, Rouzan G.
Loykasek, Patti A.
Marolt, Monna J.
Shen, Steven S.
Smith, Anthony T.
Swanson, Paul E.
[J]. ARCHIVES OF PATHOLOGY & LABORATORY MEDICINE, 2014, 138 (11) : 1432 - 1443
[9] The immune contexture in human tumours: impact on clinical outcome
Fridman, Wolf Herman
Pages, Franck
Sautes-Fridman, Catherine
Galon, Jerome
[J]. NATURE REVIEWS CANCER, 2012, 12 (04) : 298 - 306
[10] Quantitative Analysis of Immune Infiltrates in Primary Melanoma
Gartrell, Robyn D.
Marks, Douglas K.
Hart, Thomas D.
Li, Gen
Davari, Danielle R.
Wu, Alan
Blake, Zoe
Lu, Yan
Askin, Kayleigh N.
Monod, Anthea
Esancy, Camden L.
Stack, Edward C.
Jia, Dan Tong
Armenta, Paul M.
Fu, Yichun
Izaki, Daisuke
Taback, Bret
Rabadan, Raul
Kaufman, Howard L.
Drake, Charles G.
Horst, Basil A.
Saenger, Yvonne M.
[J]. CANCER IMMUNOLOGY RESEARCH, 2018, 6 (04) : 481 - 493

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