Improved isolation protocol to detect high molecular weight polysaccharide structures of Campylobacter jejuni

被引：1

作者：

Kovacs, Judit K. ^{[1
]}

Felsi, Peter ^{[1
]}

Emody, Levente ^{[1
,2
]}

Schneider, Gyoergy ^{[1
]}

Kocsis, Bela ^{[1
]}

机构：

[1] Univ Pecs, Dept Med Microbiol & Immunol, H-7624 Pecs, Hungary

[2] Hungarian Acad Sci, Agr Res Ctr, Vet Med Res Inst, Budapest, Hungary

来源：

JOURNAL OF MICROBIOLOGICAL METHODS | 2014年 / 107卷

关键词：

Polysaccharide; LPS; Silver staining; Campylobacter jejuni; LIPOPOLYSACCHARIDES;

D O I：

10.1016/j.mimet.2014.08.014

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Simple detection of high molecular weight, LPS-like structures of Campylobacter jejuni is still an unsolved problem. A phenol-free extraction method for the detection of HMW polysaccharide was developed without the need for Western blot. This method provides a reliable technique for large-scale screening and comparative characterization study of different isolates. (C) 2014 Elsevier B.V. All rights reserved.

引用

页码：55 / 57

页数：3

共 16 条

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