A new faecal antigen detection system for Strongyloides venezuelensis diagnosis in immunosuppressed rats

被引：13

作者：

Goncalves, Ana Lucia R. ^{[1
]}

Silva, Claudio V. ^{[1
]}

Ueta, Marlene T. ^{[2
]}

Costa-Cruz, Julia M. ^{[1
]}

机构：

[1] Univ Fed Uberlandia, Inst Ciencias Biomed, BR-38400902 Uberlandia, MG, Brazil

[2] Univ Estadual Campinas, Parasitol Lab, Sao Paulo, Brazil

来源：

EXPERIMENTAL PARASITOLOGY | 2010年 / 125卷 / 04期

关键词：

Strongyloides venezuelensis; Strongyloidiasis; Coproantigen; Immunosuppression; Rats; Diagnosis; LINKED-IMMUNOSORBENT-ASSAY; MHC CLASS-I; STERCORALIS INFECTION; PARASITE BURDEN; MICE; STRAINS;

D O I：

10.1016/j.exppara.2010.02.015

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

This study was performed with the objective of developing innovative procedures for the diagnosis of strongyloidiasis. Enzyme-linked immunosorbent assay (ELISA) was conducted to detect coproantigen in the faecal samples of normal and of immunosuppressed rats using an anti-L3 polyclonal antibody produced in rabbits. Analysis revealed the kinetics of egg shedding in the non-immunosuppressed and immunosuppressed rats infected with S. venezuelensis. Further analysis verified the ability of the immune serum to detect U antigens in faecal samples from infected animals. The number of eggs recovered in the faeces at 8 days p.i was significantly higher for both groups. Immunosuppressed animals eliminated increased quantities of eggs. The immune serum was able to detect 0.39 mu g/ml of L3 antigens. The antigen recognition in the immunosuppressed group was anticipated on the 8th day p.i. In conclusion, these results may represent a first step in the development of a rapid coproantigen detection kit for strongyloidiasis. (C) 2010 Elsevier Inc. All rights reserved.

引用

页码：338 / 341

页数：4

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