A membranous form of ICAM-1 on exosomes efficiently blocks leukocyte adhesion to activated endothelial cells

被引:66
作者
Lee, Hwan Myung [1 ,2 ]
Choi, Eun-Jeong [1 ,2 ]
Kim, Ji Hyun [1 ,2 ]
Kim, Thomas Doohun [3 ]
Kim, Yoon-Keun [1 ,2 ]
Kang, Chulhun [4 ]
Gho, Yong Song [1 ,2 ]
机构
[1] Pohang Univ Sci & Technol, Dept Life Sci, Pohang 790784, South Korea
[2] Pohang Univ Sci & Technol, Div Mol & Life Sci, Pohang 790784, South Korea
[3] Ajou Univ, Coll Engn, Dept Biol & Mol Engn, Suwon 443749, South Korea
[4] Kyung Hee Univ, Grad Sch EW Med Sci, Dept Neurosci, Yongin 446701, South Korea
关键词
ICAM-1; Exosomes; Microparticles; Communicasome; Inflammation; Endothelial cell; GENE-EXPRESSION; CANCER CELLS; MOLECULE-1; MICROVESICLES; VESICLES; MICROPARTICLES; DIMERIZATION; DISTINCT; SICAM-1;
D O I
10.1016/j.bbrc.2010.05.094
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
While intercellular adhesion molecule-1 (ICAM-1) is a transmembrane protein, two types of extracellular ICAM-1 have been detected in cell culture supernatants as well as in the serum: a soluble form of ICAM-1 (sICAM-1) and a membranous form of ICAM-1 (mICAM-1) associated with exosomes. Previous observations have demonstrated that sICAM-1 cannot exert potent immune modulatory activity due to its low affinity for leukocyte function-associated antigen-1 (LFA-1) or membrane attack complex-1. In this report, we initially observed that human cancer cells shed mICAM-1(+)-exosomes but were devoid of vascular cell adhesion molecule-1 and E-selectin. We demonstrate that mICAM-1 on exosomes retained its topology similar to that of cell surface ICAM-1, and could bind to leukocytes. In addition, we show that exosomal mICAM-1 exhibits potent anti-leukocyte adhesion activity to tumor necrosis factor-a-activated endothelial cells compared to that of sICAM-1. Taken together with previous findings, our results indicate that mICAM-1 on exosomes exhibits potent immune modulatory activity. (C) 2010 Elsevier Inc. All rights reserved.
引用
收藏
页码:251 / 256
页数:6
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