Identification of a Putative Novel Genotype of Avian Hepatitis E Virus from Apparently Healthy Chickens in Southwestern Nigeria

被引:5
作者
Osamudiamen, Fisayo Temilade [1 ,2 ]
Akanbi, Olusola Aanuoluwapo [2 ,4 ]
Zander, Steffen [2 ]
Oluwayelu, Daniel Oladimeji [1 ]
Bock, Claus-Thomas [2 ,3 ]
Klink, Patrycja [2 ]
机构
[1] Univ Ibadan, Dept Vet Microbiol, Ibadan 200223, Nigeria
[2] Robert Koch Inst, Div Viral Gastroenteritis & Hepatitis Pathogens &, Dept Infect Dis, D-13353 Berlin, Germany
[3] Univ Tuebigen, Inst Trop Med, D-72074 Tuebigen, Germany
[4] Nigeria Ctr Dis Control, Natl Reference Lab, Abuja 900110, Nigeria
来源
VIRUSES-BASEL | 2021年 / 13卷 / 06期
关键词
hepatitis E virus; avian HEV; layer chickens; novel genotype; COMPLETE GENOMIC SEQUENCE; SPLENOMEGALY SYNDROME; GENETIC IDENTIFICATION; ANIMAL RESERVOIRS; HEV; INFECTION; FLOCKS; DISEASE; PREVALENCE; STRAIN;
D O I
10.3390/v13060954
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Avian hepatitis E virus (aHEV) is the major etiological agent of hepatitis-splenomegaly syndrome (HSS), big liver and spleen disease (BLSD), and hepatic rupture hemorrhage syndrome (HRHS) in chickens. Infections with aHEV cause a significant decrease in egg production and increased mortality in chickens worldwide. However, studies on the prevalence of aHEV in Nigeria are scarce. In this study, serum (n = 88) and fecal samples (n = 110) obtained from apparently healthy layer chickens from three states in southwestern Nigeria were analyzed by nested reverse transcription-polymerase chain reaction (nRT-PCR) targeting the helicase and capsid gene for the presence of aHEV. Avian HEV was detected in 12.5% (n = 11/88) of serum samples and 9.1% (n = 10/110) of fecal samples tested. Phylogenetic analysis showed that five of the twelve identified aHEV sequences belonged to genotype 2. The remaining seven sequences were only distantly related to other known aHEV isolates. After amplification of the near-complete ORF2 fragment (1618 bp) and part of the ORF1 (582 bp) of isolate YF40_aHEV_NG phylogenetic analysis revealed a nucleotide sequence identity between 79.0 and 82.6% and 80.1 and 83.5%, respectively, to other known aHEV strains, indicating that the Nigerian isolate YF40_aHEV_NG belongs to a novel aHEV genotype. This is the first report of co-circulation of aHEV genotypes in chickens in Nigeria.
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页数:11
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