Comparison of platelet aggregability and P-selectin surface expression on platelets isolated by different methods

被引:49
作者
Walkowiak, B
Kralisz, U
Michalec, L
Majewska, E
Kozìolkiewicz, W
Ligocka, A
Cierniewski, CS
机构
[1] Med Univ Lodz, Dept Biophys, PL-90131 Lodz, Poland
[2] Mil Med Acad, Dept Pathophysiol, Lodz, Poland
关键词
platelets; platelet isolation; platelet activation; platelet aggregation; P-selectin; flow-cytofluorimetry;
D O I
10.1016/S0049-3848(00)00282-6
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Three methods commonly used for isolation of blood platelets from plasma were compared. Platelets were isolated by: 1) a washing method; 2) a method of metrizamide-gradient centrifugation; 3) a modified method of gel-filtration. The last method employed BSA-Sepharose gel instead of routinely used Sepharose gel saturated with BSA. BSA-Sepharose gel was prepared by covalent binding of thermally deactivated BSA to CNBr-activated Sepharose 2B. In contrast to platelets isolated by the other methods, an aggregability of the gel-filtered platelets and control platelets in plasma, both activated with ADP, were comparable. When expression of P-selectin on the surface of freshly isolated platelets was examined, the gel-filtered platelets exhibited the same extent of fluorescence signal as platelets in the citrated blood, whereas platelets isolated by the other methods exhibited twice the extent of the signal. The methods involving the centrifugation process cause a low but a significant platelet activation. (C) 2000 Elsevier Science Ltd. All rights reserved.
引用
收藏
页码:495 / 502
页数:8
相关论文
共 17 条
[1]   CHEMICAL COUPLING OF PEPTIDES AND PROTEINS TO POLYSACCHARIDES BY MEANS OF CYANOGEN HALIDES [J].
AXEN, R ;
PORATH, J ;
ERNBACK, S .
NATURE, 1967, 214 (5095) :1302-&
[2]   PROTEIN PLATELET INTERACTION WITH AN ARTIFICIAL SURFACE - EFFECT OF VITAMINS AND PLATELET INHIBITORS [J].
CHANDY, T ;
SHARMA, CP .
THROMBOSIS RESEARCH, 1986, 41 (01) :9-22
[3]   ADSORPTION OF COAGULATION PROTEINS FROM WHOLE-BLOOD ON TO POLYMER MATERIALS - RELATION TO PLATELET ACTIVATION [J].
ELAM, JH ;
NYGREN, H .
BIOMATERIALS, 1992, 13 (01) :3-8
[4]   PLATELET SURFACE GLYCOPROTEINS - STUDIES ON RESTING AND ACTIVATED PLATELETS AND PLATELET MEMBRANE MICROPARTICLES IN NORMAL SUBJECTS, AND OBSERVATIONS IN PATIENTS DURING ADULT RESPIRATORY-DISTRESS SYNDROME AND CARDIAC-SURGERY [J].
GEORGE, JN ;
PICKETT, EB ;
SAUCERMAN, S ;
MCEVER, RP ;
KUNICKI, TJ ;
KIEFFER, N ;
NEWMAN, PJ .
JOURNAL OF CLINICAL INVESTIGATION, 1986, 78 (02) :340-348
[5]  
GRYNKIEWICZ G, 1985, J BIOL CHEM, V260, P3440
[6]  
Kralisz U, 1998, BIOCHEM MOL BIOL INT, V45, P735
[7]  
MCEVER RP, 1984, J BIOL CHEM, V259, P9799
[8]  
MULVIHILL JN, 1990, J BIOMED MATER RES, V24, P153
[9]   PREPARATION OF SUSPENSIONS OF WASHED PLATELETS FROM HUMANS [J].
MUSTARD, JF ;
PACKHAM, MA ;
PERRY, DW ;
ARDLIE, NG .
BRITISH JOURNAL OF HAEMATOLOGY, 1972, 22 (02) :193-&
[10]  
*PHARM BIOT, 1994, GENEQUANT RNA DNA CA