Role of zinc along with ascorbic acid and folic acid during long-term in vitro albumin glycation

被引：36

作者：

Tupe, Rashmi Santosh ^{[1
]}

Agte, Vaishali Vilas ^{[1
]}

机构：

[1] Agharkar Res Inst, Pune 411004, Maharashtra, India

来源：

BRITISH JOURNAL OF NUTRITION | 2010年 / 103卷 / 03期

关键词：

Ascorbic acid; Bovine serum albumin; Glycation; Folic acid; Zinc; END-PRODUCTS; VITAMIN-C; SUPEROXIDE DISMUTASE; OXIDATIVE STRESS; AMYLOID PLAQUES; A-BETA; GLUCOSE; ANTIOXIDANT; COPPER; BINDING;

D O I：

10.1017/S0007114509991929

中图分类号：

R15 [营养卫生、食品卫生]; TS201 [基础科学];

学科分类号：

100403 ;

摘要：

The present study aimed to investigate the role of Zn alone and in the presence of ascorbic acid (AA) and folic acid (FA) in albumin glycation. Glycation was performed by incubations of bovine serum albumin with glucose at 37 degrees C along with Zn, AA or FA separately and Zn + AA or Zn + FA for 150d. Glycation-mediated modifications were monitored as fluorescence of advanced glycation endproducts, carbonyl formation, beta aggregation (thioflavin T and Congo red dyes), albumin-bound Zn, thiol groups and glycated aggregate's toxicity in HepG2 cells. Zn inhibited glycation and beta aggregation, probably due to observed higher protein-bound Zn. It also protected protein thiols and increased cell survival. AA and FA enhanced glycation, which was lowered in Zn-co-incubated samples. FA increased albumin-bound Zn and showed maximum cell survival. Although these results warrant further in vivo investigation, the present data help in the understanding of the interplay of Zn with micronutrients in albumin glycation.

引用

页码：370 / 377

页数：8

共 41 条

[1] Vitamin C supplementation decreases insulin glycation and improves glucose homeostasis in obese hyperglycemic (ob/ob) mice [J].

Abdel-Wahab, YHA ;

O'Harte, FPM ;

Mooney, MH ;

Barnett, CR ;

Flatt, PR .

METABOLISM-CLINICAL AND EXPERIMENTAL, 2002, 51 (04) :514-517

[2]

Afkhami-Ardekani M, 2007, INDIAN J MED RES, V126, P471

[3] Combination of diabetes and cataract worsens the oxidative stress and micronutrient status in Indians [J].

Agte, Vaishali V. ;

Tarwadi, Kirtan Vilas .

NUTRITION, 2008, 24 (7-8) :617-624

[4] Study of factors affecting binding of zinc with albumin at physiological zinc concentrations [J].

Agte, VV ;

Nagmote, RV .

BIOFACTORS, 2004, 20 (03) :139-145

[5]

Al-Maroof RA, 2006, SAUDI MED J, V27, P344

[6]

BALTZ ML, 1984, CLIN EXP IMMUNOL, V57, P657

[7] Glycation induces formation of amyloid cross-β structure in albumin [J].

Bouma, B ;

Kroon-Batenburg, LMJ ;

Wu, YP ;

Brünjes, B ;

Posthuma, G ;

Kranenburg, O ;

de Groot, PG ;

Voest, EE ;

Gebbink, MFBG .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2003, 278 (43) :41810-41819

[8] Glucose and free radicals impair the antioxidant properties of serum albumin [J].

Bourdon, E ;

Loreau, N ;

Blache, D .

FASEB JOURNAL, 1999, 13 (02) :233-244

[9] RAPID INDUCTION OF ALZHEIMER A-BETA AMYLOID FORMATION BY ZINC [J].

BUSH, AI ;

PETTINGELL, WH ;

MULTHAUP, G ;

PARADIS, MD ;

VONSATTEL, JP ;

GUSELLA, JF ;

BEYREUTHER, K ;

MASTERS, CL ;

TANZI, RE .

SCIENCE, 1994, 265 (5177) :1464-1467

[10] Toxic action of advanced glycation end products on cultured retinal capillary pericytes and endothelial cells: Relevance to diabetic retinopathy [J].

Chibber, R ;

Molinatti, PA ;

Rosatto, N ;

Lambourne, B ;

Kohner, EM .

DIABETOLOGIA, 1997, 40 (02) :156-164

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