A novel double coating for microemulsion electrokinetic chromatography with laser-induced fluorescence detection: as tested with amino acid derivatives

被引:12
作者
Xie, JP
Chen, XF
Zhang, JY
Liu, JQ
Tian, JN
Chen, XG
Hu, ZD [1 ]
机构
[1] Lanzhou Univ, Dept Chem, Lanzhou 730000, Peoples R China
[2] Zhoukou Teachers Coll, Dept Chem, Henan 466000, Peoples R China
[3] Mianyang Teachers Coll, Dept Chem, Sichuan 621000, Peoples R China
关键词
dynamic coating; double coating; hexamethyldisilazane; microemulsion electrokinetic chromatography; laser-induced fluorescence; amino acids;
D O I
10.1016/j.jpba.2004.05.004
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
A novel double coating (DC) was developed for fast and reproducible microemulsion electrokinetic chromatography (MEEKC), as tested with separation and determination of amino acids using laser-induced fluorescence (LIF) detection after derivatization with 4-chloro-7nitrobenzo-2-oxa-1,3-diazol. The simple coating is a combination of a removable covalent layer and a dynamic SDS coating. Hexamethyldisilazane was utilized for the covalent layer that can be regenerated on-line. Compared with previous no-coating method, the analysis time was shortened; and the reproducibility of migration times was improved. (C) 2004 Elsevier B.V. All rights reserved.
引用
收藏
页码:1 / 8
页数:8
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