A novel double coating for microemulsion electrokinetic chromatography with laser-induced fluorescence detection: as tested with amino acid derivatives

被引：12

作者：

Xie, JP

Chen, XF

Zhang, JY

Liu, JQ

Tian, JN

Chen, XG

Hu, ZD ^{[1
]}

机构：

[1] Lanzhou Univ, Dept Chem, Lanzhou 730000, Peoples R China

[2] Zhoukou Teachers Coll, Dept Chem, Henan 466000, Peoples R China

[3] Mianyang Teachers Coll, Dept Chem, Sichuan 621000, Peoples R China

来源：

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS | 2004年 / 36卷 / 01期

关键词：

dynamic coating; double coating; hexamethyldisilazane; microemulsion electrokinetic chromatography; laser-induced fluorescence; amino acids;

D O I：

10.1016/j.jpba.2004.05.004

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A novel double coating (DC) was developed for fast and reproducible microemulsion electrokinetic chromatography (MEEKC), as tested with separation and determination of amino acids using laser-induced fluorescence (LIF) detection after derivatization with 4-chloro-7nitrobenzo-2-oxa-1,3-diazol. The simple coating is a combination of a removable covalent layer and a dynamic SDS coating. Hexamethyldisilazane was utilized for the covalent layer that can be regenerated on-line. Compared with previous no-coating method, the analysis time was shortened; and the reproducibility of migration times was improved. (C) 2004 Elsevier B.V. All rights reserved.

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页码：1 / 8

页数：8

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