A novel double coating for microemulsion electrokinetic chromatography with laser-induced fluorescence detection: as tested with amino acid derivatives
被引:12
作者:
Xie, JP
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机构:Lanzhou Univ, Dept Chem, Lanzhou 730000, Peoples R China
Xie, JP
Chen, XF
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机构:Lanzhou Univ, Dept Chem, Lanzhou 730000, Peoples R China
Chen, XF
Zhang, JY
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机构:Lanzhou Univ, Dept Chem, Lanzhou 730000, Peoples R China
Zhang, JY
Liu, JQ
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机构:Lanzhou Univ, Dept Chem, Lanzhou 730000, Peoples R China
Liu, JQ
Tian, JN
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机构:Lanzhou Univ, Dept Chem, Lanzhou 730000, Peoples R China
Tian, JN
Chen, XG
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机构:Lanzhou Univ, Dept Chem, Lanzhou 730000, Peoples R China
Chen, XG
Hu, ZD
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Lanzhou Univ, Dept Chem, Lanzhou 730000, Peoples R ChinaLanzhou Univ, Dept Chem, Lanzhou 730000, Peoples R China
Hu, ZD
[1
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机构:
[1] Lanzhou Univ, Dept Chem, Lanzhou 730000, Peoples R China
[2] Zhoukou Teachers Coll, Dept Chem, Henan 466000, Peoples R China
[3] Mianyang Teachers Coll, Dept Chem, Sichuan 621000, Peoples R China
A novel double coating (DC) was developed for fast and reproducible microemulsion electrokinetic chromatography (MEEKC), as tested with separation and determination of amino acids using laser-induced fluorescence (LIF) detection after derivatization with 4-chloro-7nitrobenzo-2-oxa-1,3-diazol. The simple coating is a combination of a removable covalent layer and a dynamic SDS coating. Hexamethyldisilazane was utilized for the covalent layer that can be regenerated on-line. Compared with previous no-coating method, the analysis time was shortened; and the reproducibility of migration times was improved. (C) 2004 Elsevier B.V. All rights reserved.
机构:
Pharmaceut Dev Europe, GlaxoSmithKline Res & Dev, Ware SG12 0DP, Herts, EnglandPharmaceut Dev Europe, GlaxoSmithKline Res & Dev, Ware SG12 0DP, Herts, England
机构:
Glaxo Wellcome Res & Dev Ltd, Pharmaceut Dev, Ware SG12 0DP, Herts, EnglandGlaxo Wellcome Res & Dev Ltd, Pharmaceut Dev, Ware SG12 0DP, Herts, England
机构:City Univ Hong Kong, Dept Biol & Chem, Hong Kong, Hong Kong, Peoples R China
Hu, S
;
Li, PCH
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机构:
City Univ Hong Kong, Dept Biol & Chem, Hong Kong, Hong Kong, Peoples R ChinaCity Univ Hong Kong, Dept Biol & Chem, Hong Kong, Hong Kong, Peoples R China
机构:
Pharmaceut Dev Europe, GlaxoSmithKline Res & Dev, Ware SG12 0DP, Herts, EnglandPharmaceut Dev Europe, GlaxoSmithKline Res & Dev, Ware SG12 0DP, Herts, England
机构:
Glaxo Wellcome Res & Dev Ltd, Pharmaceut Dev, Ware SG12 0DP, Herts, EnglandGlaxo Wellcome Res & Dev Ltd, Pharmaceut Dev, Ware SG12 0DP, Herts, England
机构:City Univ Hong Kong, Dept Biol & Chem, Hong Kong, Hong Kong, Peoples R China
Hu, S
;
Li, PCH
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机构:
City Univ Hong Kong, Dept Biol & Chem, Hong Kong, Hong Kong, Peoples R ChinaCity Univ Hong Kong, Dept Biol & Chem, Hong Kong, Hong Kong, Peoples R China