Long noncoding RNA NEAT1 promotes laryngeal squamous cell cancer through regulating miR-107/CDK6 pathway

被引：152

作者：

Wang, Peng ^{[1
]}

Wu, Tianyi ^{[1
]}

Zhou, Han ^{[1
]}

Jin, Qianqian ^{[1
]}

He, Guoqing ^{[1
]}

Yu, Haoyang ^{[1
]}

Xuan, Lijia ^{[1
]}

Wang, Xin ^{[1
]}

Tian, Linli ^{[1
]}

Sun, Yanan ^{[1
]}

Liu, Ming ^{[1
]}

Qu, Lingmei ^{[2
]}

机构：

[1] Harbin Med Univ, Affiliated Hosp 2, Dept Otorhinolaryngol Head & Neck Surg, 246 Baojian Rd, Harbin 150086, Peoples R China

[2] Harbin Med Univ, Affiliated Hosp 5, Dept Otorhinolaryngol Head & Neck Surg, 213 Jianshe Rd, Daqing, Peoples R China

来源：

JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH | 2016年 / 35卷

基金：

美国国家科学基金会;

关键词：

Laryngeal squamous cell cancer; Long noncoding RNA; microRNA; NEAT1; CDK6; miR-107; DOWN-REGULATION; FUNCTIONAL-ROLE; CARCINOMA; EXPRESSION; APOPTOSIS; PROLIFERATION; INVASION; HOTAIR; MIR-21; GROWTH;

D O I：

10.1186/s13046-016-0297-z

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Background: Long noncoding RNA nuclear paraspeckle assembly transcript 1 (NEAT1) plays key role in the progression of some human cancers. However, the role of NEAT1 in human laryngeal squamous cell cancer (LSCC) is still unknown. We therefore investigated the expression and function of NEAT1 in LSCC. Methods: NEAT1 level in LSCC and adjacent non-neoplastic tissues were detected by qRT-PCR. NEAT1 was knockdown in LSCC cells and cell proliferation, apoptosis and cell cycle were examined. The growth of xenografts with NEAT1 knockdown LSCC cells was analyzed. Results: NEAT1 level was significantly higher in LSCC than in corresponding adjacent non-neoplastic tissues, and patients with neck nodal metastasis or advanced clinical stage had higher NEAT1 expression. Moreover, siRNA mediated NEAT1 knockdown significantly inhibited the proliferation and induced apoptosis and cell cycle arrest at G1 phase in LSCC cells. The growth of LSCC xenografts was significantly suppressed by the injection of NEAT1 siRNA lentivirus. Furthermore, NEAT1 regulated CDK6 expression in LSCC cells which was mediated by miR-107. Conclusion: NEAT1 plays an oncogenic role in the tumorigenesis of LSCC and may serve as a potential target for therapeutic intervention.

引用

页数：11

共 27 条

[1] The oestrogen receptor alpha-regulated lncRNA NEAT1 is a critical modulator of prostate cancer [J].