Chemoattractant receptor-stimulated F-actin polymerization in the human neutrophil is signaled by 2 distinct pathways

被引：60

作者：

Chodniewicz, D ^{[1
]}

Zhelev, DV ^{[1
]}

机构：

[1] Duke Univ, Dept Mech Engn & Mat Sci, Durham, NC 27708 USA

来源：

BLOOD | 2003年 / 101卷 / 03期

关键词：

D O I：

10.1182/blood-2002-05-1435

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

We characterized the overall rate of F-actin polymerization in the pseudopod region by measuring the rate of extension of single pseudopods stimulated by f-Met-Leu-Phe. The rate of pseudopod extension was measured in the presence of inhibitors for signaling molecules that are known to be involved motility. Our data show the existence of 2 distinct signaling pathways of actin polymerization in the pseudopod region: a phosphoinositide 3-kinase gamma (P13Kgamma)-dependent and -independent pathway. The P13Kgamma dependent signaling of F-actin polymerization also depends on protein kinase C zeta and protein kinase B (Akt/PKB). The P13Kgamma-independent pathway depends on GTPase RhoA, the RhoA ROCK kinase, Src family tyrosine kinases, and NADPH, and is modulated by cAMP. (C) 2003 by The American Society of Hematology.

引用

页码：1181 / 1184

页数：4

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