Risk of horizontal transfer of intracellular, extracellular, and bacteriophage antibiotic resistance genes during anaerobic digestion of cow manure

被引：24

作者：

Xu, Liang ^{[1
]}

Gu, Jie ^{[1
,2
]}

Wang, Xiaojuan ^{[1
,2
]}

Song, Zilin ^{[1
,2
]}

Jiang, Haihong ^{[1
]}

Li, Nana ^{[3
]}

Lei, Liusheng ^{[1
]}

Xie, Jun ^{[1
]}

Hu, Ting ^{[1
]}

Ding, Qingling ^{[1
]}

Sun, Yifan ^{[1
]}

机构：

[1] Northwest A&F Univ, Coll Nat Resources & Environm, Yangling 712100, Shaanxi, Peoples R China

[2] Northwest A&F Univ, Shaanxi Engn Res Ctr Utilizat Agr Waste Resources, Yangling 712100, Shaanxi, Peoples R China

[3] Shaanxi Normal Univ, Sch Geog & Tourism, Xian 710119, Shaanxi, Peoples R China

来源：

BIORESOURCE TECHNOLOGY | 2022年 / 351卷

基金：

中国博士后科学基金;

关键词：

Bacteriophage antibiotic resistance genes; Extracellular antibiotic resistance genes; Hosts; Mesophilic anaerobic digestion; Thermophilic anaerobic digestion; WASTE-WATER; BACTERIAL COMMUNITY; SWINE MANURE; FATE; DISSEMINATION; ABUNDANCE; TEMPERATURE; PERSISTENCE; DIVERSITY; EVOLUTION;

D O I：

10.1016/j.biortech.2022.127007

中图分类号：

S2 [农业工程];

学科分类号：

0828 ;

摘要：

The fate of intracellular antibiotic resistance genes (iARGs), extracellular ARGs (eARGs) and bacteriophage ARGs (bARGs) during anaerobic digestion (AD) of cow manure is unclear. Thus, the characteristics of iARGs, eARGs and bARGs during mesophilic AD (MAD) and thermophilic AD (TAD) of cow manure were investigated. The absolute abundances of iARGs decreased by 69.82% after TAD. After MAD and TAD, the total absolute abundances of eARGs increased by 63.5 times and 67.6 times, respectively, whereas those of the bARGs increased by 47.60% and 59.22%. eARGs were mainly derived from the non-specific lysis of Firmicutes, Bacteroidetes, while bacteriophages had a wide range of hosts. The variations in iARGs, eARGs and bARGs were affected by the microbial hosts but also directly driven by physicochemical factors (e.g., pH). Overall, the findings of this study revealed that there may be a risk of eARGs and bARGs disseminating during the AD of cow manure.

引用

页数：10

共 49 条

[1] Persistence of naturally occurring antibiotic resistance genes in the bacteria and bacteriophage fractions of wastewater [J].

Calero-Caceres, William ;

Muniesa, Maite .

WATER RESEARCH, 2016, 95 :11-18

[2] Influence of temperature on volatile fatty acid production and microbial community structure during anaerobic fermentation of microalgae [J].

Cho, Hyun Uk ;

Kim, Young Mo ;

Choi, Yun-Nam ;

Kim, Hye Gyeong ;

Park, Jong Moon .

BIORESOURCE TECHNOLOGY, 2015, 191 :475-480

[3] Viruses as key reservoirs of antibiotic resistance genes in the environment [J].

Debroas, Didier ;

Siguret, Clea .

ISME JOURNAL, 2019, 13 (11) :2856-2867

[4] Whole rumen metagenome sequencing allows classifying and predicting feed efficiency and intake levels in cattle [J].

Delgado, Beatriz ;

Bach, Alex ;

Guasch, Isabel ;

Gonzalez, Carmen ;

Elcoso, Guillermo ;

Pryce, Jennie E. ;

Gonzalez-Recio, Oscar .

SCIENTIFIC REPORTS, 2019, 9 (1)

[5] Natural Transformation Facilitates Transfer of Transposons, Integrons and Gene Cassettes between Bacterial Species [J].

Domingues, Sara ;

Harms, Klaus ;

Fricke, W. Florian ;

Johnsen, Pal J. ;

da Silva, Gabriela J. ;

Nielsen, Kaare Magne .

PLOS PATHOGENS, 2012, 8 (08)

[6] Assessment of extracellular antibiotic resistance genes (eARGs) in typical environmental samples and the transforming ability of eARG [J].

Dong, Peiyan ;

Wang, Hui ;

Fang, Tingting ;

Wang, Yun ;

Ye, Quanhui .

ENVIRONMENT INTERNATIONAL, 2019, 125 :90-96

[7] Responses of bacterial and bacteriophage communities to long-term exposure to antimicrobial agents in wastewater treatment systems [J].

Du, Bingbing ;

Wang, Qiang ;

Yang, Qingxiang ;

Wang, Ruifei ;

Yuan, Wei ;

Yan, Luyu .

JOURNAL OF HAZARDOUS MATERIALS, 2021, 414

[8] Prevalence of Antibiotic Resistance Genes and Bacterial Pathogens in Long-Term Manured Greenhouse Soils As Revealed by Metagenomic Survey [J].

Fang, Hua ;

Wang, Huifang ;

Cai, Lin ;

Yu, Yunlong .

ENVIRONMENTAL SCIENCE & TECHNOLOGY, 2015, 49 (02) :1095-1104

[9] The role of anaerobic digestion in controlling the release of tetracycline resistance genes and class 1 integrons from municipal wastewater treatment plants [J].

Ghosh, Sudeshna ;

Ramsden, Sara J. ;

LaPara, Timothy M. .

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2009, 84 (04) :791-796

[10] Dissemination of Antibiotic Resistance Genes in Representative Broiler Feedlots Environments: Identification of Indicator ARGs and Correlations with Environmental Variables [J].

He, Liang-Ying ;

Liu, You-Sheng ;

Su, Hao-Chang ;

Zhao, Jian-Liang ;

Liu, Shuang-Shuang ;

Chen, Jun ;

Liu, Wang-Rong ;

Ying, Guang-Guo .

ENVIRONMENTAL SCIENCE & TECHNOLOGY, 2014, 48 (22) :13120-13129

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