Adenosine promotes neuronal recovery from reactive oxygen species induced lesion in rat hippocampal slices

被引：45

作者：

Almeida, CG ^{[1
]}

de Mendonça, A ^{[1
]}

Cunha, RA ^{[1
]}

Ribeiro, JA ^{[1
]}

机构：

[1] Fac Med Lisbon, Neurosci Lab, P-1649028 Lisbon, Portugal

来源：

NEUROSCIENCE LETTERS | 2003年 / 339卷 / 02期

关键词：

adenosine; neuroprotection; reactive oxygen species; hippocampus; A(1) receptors; A(2A) receptors; dipropylcyclopentylxanthine;

D O I：

10.1016/S0304-3940(02)01478-7

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Reactive oxygen species (ROS) are believed to be involved in the pathogenesis of several neurological disorders. We now tested whether the endogenous neuroprotective substance, adenosine, attenuates the cell damage induced by ROS. In rat hippocampal slices, the xanthine oxidase (40 mU/ml) plus xanthine (I mM) (X/XO) system produced a 27.8 +/- 7.3% (n = 3) increase in ROS, measured by fluorimetry with 2',7'-dichlorodihydrofluorescein, a 246.9 +/- 18.4% (n = 6) increase in the release of tritiated adenosine, and a decrease in synaptic transmission that fully recovered after washout. In the presence of the adenosine A, receptor selective antagonist, 1,3-dipropyl-8-cyclopentylxanthine (100 nM), X/XO induced a similar inhibition, however synaptic transmission only recovered to 70.7 +/- 5.8% of control (n = 5). The blockade of A(2A) receptors was devoid of effect (it = 4). Adenosine is released by ROS-generating systems, and attenuates the deleterious cellular consequences of ROS through A, receptor activation. (C) 2003 Elsevier Science Ireland Ltd. All rights reserved.

引用

页码：127 / 130

页数：4

共 23 条

[1] The LTP Program: a data acquisition program for on-line analysis of long-term potentiation and other synaptic events [J].

Anderson, WW ;

Collingridge, GL .

JOURNAL OF NEUROSCIENCE METHODS, 2001, 108 (01) :71-83

[2] AGING, ENERGY, AND OXIDATIVE STRESS IN NEURODEGENERATIVE DISEASES [J].

BEAL, MF .

ANNALS OF NEUROLOGY, 1995, 38 (03) :357-366

[3] Nitric oxide interacts with oxygen free radicals to evoke the release of adenosine and adenine nucleotides from rat hippocampal slices [J].

Broad, RM ;

Fallahi, N ;

Fredholm, BB .

JOURNAL OF THE AUTONOMIC NERVOUS SYSTEM, 2000, 81 (1-3) :82-86

[4] REGULATION OF GLUTAMATE AND ASPARTATE RELEASE FROM SLICES OF THE HIPPOCAMPAL CA1 AREA - EFFECTS OF ADENOSINE AND BACLOFEN [J].

BURKE, SP ;

NADLER, JV .

JOURNAL OF NEUROCHEMISTRY, 1988, 51 (05) :1541-1551

[5] OXYGEN FREE-RADICAL INVOLVEMENT IN ISCHEMIA AND REPERFUSION INJURY TO BRAIN [J].

CAO, W ;

CARNEY, JM ;

DUCHON, A ;

FLOYD, RA ;

CHEVION, M .

NEUROSCIENCE LETTERS, 1988, 88 (02) :233-238

[6] THE EFFECT OF XANTHINE/XANTHINE OXIDASE GENERATED REACTIVE OXYGEN SPECIES ON SYNAPTIC TRANSMISSION [J].

COLTON, C ;

YAO, J ;

GROSSMAN, Y ;

GILBERT, D .

FREE RADICAL RESEARCH COMMUNICATIONS, 1991, 14 (5-6) :385-393

[7] Adenosine as a neuromodulator and as a homeostatic regulator in the nervous system: different roles, different sources and different receptors [J].

Cunha, RA .

NEUROCHEMISTRY INTERNATIONAL, 2001, 38 (02) :107-125

[8]

Cunha RA, 1996, J NEUROCHEM, V67, P2180

[9] Adenosine:: does it have a neuroprotective role after all? [J].

de Mendonça, A ;

Sebastiao, AM ;

Ribeiro, JA .

BRAIN RESEARCH REVIEWS, 2000, 33 (2-3) :258-274

[10] The role and regulation of adenosine in the central nervous system [J].

Dunwiddie, TV ;

Masino, SA .

ANNUAL REVIEW OF NEUROSCIENCE, 2001, 24 :31-55

← 1 2 3 →