Stromal factors support the expansion of the whole hemopoietic spectrum from bone marrow CD34+DR- cells and of some hemopoietic subsets from CD34+ and CD34+DR+ cells

Ex vivo expanded bone marrow CD34(+)DR(-) cells could offer a graft devoid of malignant cells able to promptly reconstitute hemopoiesis after transplant. We investigated the specific expansion requirements of this subpopulation compared to the more mature CD34(+) and CD34(+)DR(+) populations. The role of stromal factors was assessed by comparing the expansion obtained when the cells were cultured in (1) long-term bone marrow culture (LTBMC) medium conditioned by an irradiated human BM stroma (CM), (2) medium supplemented with 15% FBS (FBSM) and(3) non-conditioned LTBMC medium (LTM) for 21 days. The effect of the addition of G-CSF (G) and/or of MIP-1 alpha (M) to a combination of IL-3, SCF, IL-6 and IL-11 (3, S, 6, II) was analyzed. Compared to CD34(+)DR(-) cells, CD34(+) and CD34(+)DR(+) cells gave rise to a similar number of viable cells and to a lower progenitor expansion. The expansion potential of CD34(+) and CD34(+)DR(+) cells was equivalent in CM and in FBSM except for both the emergence of CD61(+) megakaryocytic cells and LTC-IC maintenance which were improved by culture in CM. In contrast, expansion from CD34(+)DR(-) cells was enhanced by CM for all the parameters tested. Compared to FBSM, CM induced a higher revel of CFU-GM and BFU-E expansion and allowed the emergence of CD61(+) cells. HPP-CFC were maintained or expanded in CM but decreased in FBSM. Compared to input, the number of LTC-IC remaining after 21 days of CD34(+)DR(-) expansion culture was strongly decreased in FBSM and variably maintained or expanded in CM. Comparison with LTM indicated that stroma conditioning is responsible for this effect. G-CSF significantly improved CFU-GM and HPP-CFC expansion from CD34(+)DR(-) cells without being detrimental to the LTC-IC pool. The growth of CD61(+) cells was significantly enhanced by G-CSF in CM. Addition of MIP-1 alpha, had no significant effect either on progenitor expansion or on LTC-IC, regardless of culture medium. We conclude that factors present in stroma-conditioned medium are necessary to support the expansion of the whole spectrum of hematopoietic cells from CD34(+)DR(-) cells and to support the expansion of cell subsets from CD34(+) and CD34(+)DR(+).