Characterization of class I integrons in clinical strains of Salmonella enterica subsp enterica serovars Typhimurium and Enteritidis from Norwegian hospitals

被引:62
作者
Lindstedt, BA [1 ]
Heir, E
Nygård, I
Kapperuld, G
机构
[1] Norwegian Inst Publ Hlth, Div Infect Dis Control, N-0403 Oslo, Norway
[2] Norwegian Sch Vet Med, Dept Pharmacol, N-0033 Oslo, Norway
关键词
D O I
10.1099/jmm.0.04958-0
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The characterization of integrons and their promoters in 156 anti biotic-resistant clinical isolates of the important zoonotic pathogens Salmonella enterica subsp. enterica serovars Typhimurium (S. Typhimurium) and Enteritidis (S. Enteritidis) from Norwegian hospitals were performed. Integrons were found in 64 of 66 S. Typhimurium isolates (97 %) and in 20 of 90 S. Enteritidis isolates (22.2 %) with the following sizes; 650, 1000, 1200, 1500, 1600, 1700, 2000 and 2100 bp. The integrons were further sequenced and the aadA1, aadA2, aadA5, aadB, pse-1, catB3, oxa1, dfrA1, dfrA12 and dfrA17 genes, as well as a fragment of the salt1 gene, were found embedded in cassettes. An internal fragment of the purG gene was additionally found as an artefact PCR amplicon.
引用
收藏
页码:141 / 149
页数:9
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共 42 条
[1]   Epidemiology -: Tracing the origins of Salmonella outbreaks [J].
Bäumler, AJ ;
Hargis, BM ;
Tsolis, RM .
SCIENCE, 2000, 287 (5450) :50-52
[2]   REVISED ANALYSIS OF AADA2 GENE OF PLASMID PSA [J].
BITO, A ;
SUSANI, M .
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1994, 38 (05) :1172-1175
[3]   Molecular characterization of an antibiotic resistance gene cluster of Salmonella typhimurium DT104 [J].
Briggs, CE ;
Fratamico, PM .
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1999, 43 (04) :846-849
[4]   Detection and characterisation of integrons in Salmonella enterica serotype Enteritidis [J].
Brown, AW ;
Rankin, SC ;
Platt, DJ .
FEMS MICROBIOLOGY LETTERS, 2000, 191 (01) :145-149
[5]   The integrons In0, In2, and In5 are defective transposon derivatives [J].
Brown, HJ ;
Stokes, HW ;
Hall, RM .
JOURNAL OF BACTERIOLOGY, 1996, 178 (15) :4429-4437
[6]   NEW MOBILE GENE CASSETTES CONTAINING AN AMINOGLYCOSIDE RESISTANCE GENE, AACA7, AND A CHLORAMPHENICOL RESISTANCE GENE, CATB3, IN AN INTEGRON IN PBWH301 [J].
BUNNY, KL ;
HALL, RM ;
STOKES, HW .
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1995, 39 (03) :686-693
[7]   Expanding drug resistance through integron acquisition by IncFI plasmids of Salmonella enterica typhimurium [J].
Carattoli, A ;
Villa, L ;
Pezzella, C ;
Bordi, E ;
Visca, P .
EMERGING INFECTIOUS DISEASES, 2001, 7 (03) :444-447
[8]   Characterisation of drug resistance gene cassettes associated with class 1 integrons in clinical isolates of Escherichia coli from Taiwan, ROC [J].
Chang, CY ;
Chang, LL ;
Chang, YH ;
Lee, TM ;
Chang, SF .
JOURNAL OF MEDICAL MICROBIOLOGY, 2000, 49 (12) :1097-1102
[9]   Two new gene cassettes, drf17 (for trimethoprim resistance) and aadA4 (for spectinomycin/streptomycin resistance), inserted in an Escherichia coli class 1 integron [J].
Chang, CY ;
Chang, LL ;
Chang, YH ;
Lee, TM ;
Li, YH ;
Chang, SF .
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 2000, 46 (01) :87-89
[10]   EXPRESSION OF ANTIBIOTIC-RESISTANCE GENES IN THE INTEGRATED CASSETTES OF INTEGRONS [J].
COLLIS, CM ;
HALL, RM .
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 1995, 39 (01) :155-162