Tissue engineering approach to repair abdominal wall defects using cell-seeded bovine tunica vaginalis in a rabbit model

被引：25

作者：

Ayele, T. ^{[1
,2
]}

Zuki, A. B. Z. ^{[1
]}

Noorjahan, B. M. A. ^{[3
]}

Noordin, M. M. ^{[1
]}

机构：

[1] Univ Putra Malaysia, Fac Vet Med, Serdang 43400, Darul Ehsan, Malaysia

[2] Univ Gondar, Fac Vet Med, Gondar, Ethiopia

[3] Univ Putra Malaysia, Fac Biotechnol & Bimol Sci, Serdang 43400, Darul Ehsan, Malaysia

来源：

JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE | 2010年 / 21卷 / 05期

关键词：

SMALL-INTESTINAL SUBMUCOSA; ACELLULAR MATRIX; MUSCLE; GRAFT; BIOMATERIALS; IMPLANTATION; PERICARDIUM; MYOBLASTS;

D O I：

10.1007/s10856-010-4007-7

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

The aim of this study was to engineer skeletal muscle tissue for repair abdominal wall defects. Myoblast were seeded onto the scaffolds and cultivated in vitro for 5 days. Full thickness abdominal wall defects (3 x 4 cm) were created in 18 male New Zealand white rabbits and randomly divided into two equal groups. The defects of the first group were repaired with myoblast-seeded-bovine tunica vaginalis whereas the second group repaired with non-seeded-bovine tunica vaginalis and function as a control. Three animals were sacrificed at 7th, 14th, and 30th days of post-implantation from each group and the explanted specimens were subjected to macroscopic and microscopic analysis. In every case, seeded scaffolds have better deposition of newly formed collagen with neo-vascularisation than control group. Interestingly, multinucleated myotubes and myofibers were only detected in cell-seeded group. This study demonstrated that myoblast-seeded-bovine tunica vaginalis can be used as an effective scaffold to repair severe and large abdominal wall defects with regeneration of skeletal muscle tissue.

引用

页码：1721 / 1730

页数：10

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