3D-Non-destructive Imaging through Heavy-Metal Eosin Salt Contrast Agents

被引：10

作者：

Busse, Madleen ^{[1
,2
]}

Marciniszyn, Jaroslaw P. ^{[3
,4
]}

Ferstl, Simone ^{[1
,2
]}

Kimm, Melanie A. ^{[5
]}

Pfeiffer, Franz ^{[1
,2
,5
]}

Gulder, Tanja ^{[3
,4
,6
]}

机构：

[1] Tech Univ Munich, Dept Phys, D-85748 Garching, Germany

[2] Tech Univ Munich, Munich Sch Bioengn, D-85748 Garching, Germany

[3] Tech Univ Munich, Dept Chem, D-85748 Garching, Germany

[4] Tech Univ Munich, Catalysis Res Ctr CRC, D-85748 Garching, Germany

[5] Tech Univ Munich, Dept Diagnost & Intervent Radiol, Klinikum Rechts Isar, D-81675 Munich, Germany

[6] Univ Leipzig, Inst Organ Chem, D-04103 Leipzig, Germany

来源：

CHEMISTRY-A EUROPEAN JOURNAL | 2021年 / 27卷 / 14期

基金：

欧盟地平线“2020”;

关键词：

dyes; eosin Y; fluorescein; microCT; stain; COMPUTED-TOMOGRAPHY; 3-D RECONSTRUCTION; SERIAL SECTIONS; MICROSCOPY; ALIGNMENT;

D O I：

10.1002/chem.202005203

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Conventional histology is a destructive technique based on the evaluation of 2D slices of a 3D biopsy. By using 3D X-ray histology these obstacles can be overcome, but their application is still restricted due to the inherently low attenuation properties of soft tissue. In order to solve this problem, the tissue can be stained before X-ray computed tomography imaging (CT) to enhance the soft tissue X-ray contrast. Evaluation of brominated fluorescein salts revealed a mutual influence of the number of bromine atoms and the cations applied on the achieved contrast enhancement. The dibromo fluorescein barium salt turned out to be the ideal X-ray contrast agent, allowing for 3D imaging and subsequent complementing counterstaining applying standard histological techniques.

引用

页码：4561 / 4566

页数：6

共 37 条

[11]

Hale IL, 2002, METHOD CELL BIOL, V70, P301

[12] Synthetic applications of eosin Y in photoredox catalysis [J].

Hari, Durga Prasad ;

Koenig, Burkhard .

CHEMICAL COMMUNICATIONS, 2014, 50 (51) :6688-6699

[13] An eosin Y method for protein determination in solution [J].

Hong, HY ;

Yoo, GS ;

Choi, JK .

ANALYTICAL LETTERS, 1999, 32 (12) :2427-2442

[14] Micro-computed tomography with iodine staining resolves the arrangement of muscle fibres [J].

Jeffery, Nathan S. ;

Stephenson, Robert S. ;

Gallagher, James A. ;

Jarvis, Jonathan C. ;

Cox, Philip G. .

JOURNAL OF BIOMECHANICS, 2011, 44 (01) :189-192

[15] Contrast-Enhanced Nanofocus X-Ray Computed Tomography Allows Virtual Three-Dimensional Histopathology and Morphometric Analysis of Osteoarthritis in Small Animal Models [J].

Kerckhofs, Greet ;

Sainz, Julie ;

Marechal, Marina ;

Wevers, Martine ;

Van de Putte, Tom ;

Geris, Liesbet ;

Schrooten, Jan .

CARTILAGE, 2014, 5 (01) :55-65

[16] The History and Future of X-ray Microscopy [J].

Kirz, J. ;

Jacobsen, C. .

9TH INTERNATIONAL CONFERENCE ON X-RAY MICROSCOPY, 2009, 186

[17]

Metscher Brian D., 2009, BMC Physiology, V9, P11, DOI 10.1186/1472-6793-9-11

[18] MicroCT for Developmental Biology: A Versatile Tool for High-Contrast 3D Imaging at Histological Resolutions [J].

Metscher, Brian D. .

DEVELOPMENTAL DYNAMICS, 2009, 238 (03) :632-640

[19] 3-DIMENSIONAL RECONSTRUCTION - A TISSUE EMBEDDING METHOD FOR ALIGNMENT OF SERIAL SECTIONS [J].

MEYER, EP ;

DOMANICO, VJ .

JOURNAL OF NEUROSCIENCE METHODS, 1988, 26 (02) :129-132

[20] X-ray microtomography in biology [J].

Mizutani, Ryuta ;

Suzuki, Yoshio .

MICRON, 2012, 43 (2-3) :104-115

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