Enantioseparation of N-methyl duloxetine, duloxetine, and fluoxetine by countercurrent chromatography using anionic β-cyclodextrin as chiral selector

被引：4

作者：

Sun, Wenyu ^{[1
]}

Chen, Ben ^{[1
]}

You, Haibo ^{[1
]}

Fang, Liqun ^{[1
]}

Qian, Junqing ^{[1
]}

Tong, Shengqiang ^{[1
]}

机构：

[1] Zhejiang Univ Technol, Coll Pharmaceut Sci, Chaowang Rd 18,Chaohui 6, Hangzhou, Peoples R China

来源：

JOURNAL OF SEPARATION SCIENCE | 2022年 / 45卷 / 15期

基金：

中国国家自然科学基金;

关键词：

anionic beta-cyclodextrins; antidepressant; countercurrent chromatography; enantioseparation; ENANTIOMERIC SEPARATION; CAPILLARY-ELECTROPHORESIS; RESOLUTION; SEROTONIN; NORFLUOXETINE; OPTIMIZATION; INHIBITOR; SYSTEM;

D O I：

10.1002/jssc.202200151

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Two anionic beta-cyclodextrins as chiral selectors were successfully applied in the enantioseparation of N-methyl duloxetine, duloxetine, and fluoxetine by countercurrent chromatography. Sulfobutyl ether-beta-cyclodextrin and carboxymethyl-beta-cyclodextrin showed opposite enantioselectivity for both duloxetine and N-methyl duloxetine enantiomers. Two biphasic solvent systems, n-hexane: 0.1 mol/L phosphate buffer pH 7.6 with 50 mmol/L of sulfobutyl ether-beta-cyclodextrin (1:1, v/v) and n-hexane: 0.1 mol/L phosphate buffer pH 7.2 with 50 mmol/L of carboxymethyl-beta-cyclodextrin (1:1, v/v), were selected for N-methyl duloxetine. Enantioseparation of duloxetine was achieved by recycling countercurrent chromatography using a solvent system composed of n-butyl acetate: 0.1 mol/L phosphate buffer pH 7.2 with 20 mmol/L of sulfobutyl ether-beta-cyclodextrin or carboxymethyl-beta-cyclodextrin (1:1, v/v). Asolvent system composed of n-hexane: n-butyl acetate: 0.1 mol/L phosphate buffer pH 7.6 containing 20 mmol/L of sulfobutyl ether-beta-cyclodextrin (6:4:10, v/v) was selected for enantioseparation of fluoxetine.

引用

页码：3022 / 3030

页数：9